NDLI: Polo-like kinase 1 phosphorylation of p150Glued facilitates nuclear envelope breakdown during prophase

Content Provider	PubMed Central
Author	Li, Hongchang Liu, X. Shawn Yang, Xiaoming Song, Bing Wang, Yun Liu, Xiaoqi
Abstract	Nuclear envelope breakdown (NEBD) is an essential step during the G2/M transition in higher eukaryotic cells. Increasing evidence supports the notion that both microtubules and microtubule-associated motor proteins are critical regulators of NEBD. Although it has been described that p150Glued, the major component of the dynein/dynactin complex, localizes in the nuclear envelope during prophase, the exact role of p150Glued and its regulation during NEBD are largely elusive. Polo-like kinase 1 (Plk1), the best characterized Ser/Thr kinase, is involved in mitotic entry in several systems; however, the targets of Plk1 during NEBD are unknown. Herein, we show that in mammalian cells both Plk1 and p150Glued regulate NEBD and that Plk1 interacts with and phosphoryates p150Glued during NEBD at prophase. Using various approaches, we showed that Plk1 phosphorylates p150Glued at Ser-179 and that the pS179 epitope is generated at the nuclear envelope of prophase cells. Significantly, Plk1-mediated phosphorylation of p150Glued at Ser-179 positively regulates its accumulation at the nuclear envelope during prophase. Finally, we found that cells expressing the Plk1-unphosphorylatable mutant (p150Glued-S179A) arrest at G2, as indicated by reduced NEBD, increased levels of cyclin B and phospho-H3, but a decreased level of Cdc2 kinase activity. Taking these data together, we conclude that Plk1 phosphorylation of p150Glued might be one major pathway of NEBD regulation.
Related Links	http://dx.doi.org/10.1073/pnas.1006615107
Ending Page	14638
Page Count	6
Starting Page	14633
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	33
Volume Number	107
Language	English
Publisher	National Academy of Sciences
Publisher Date	2010-08-17
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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