NDLI: 2D-PCR: a method of mapping DNA in tissue sections†

Content Provider	PubMed Central
Author	Armani, Michael Jaime, Rodriguez-canales Gillespie, John Michael, Tangrea Erickson, Heidi Emmert-buck, Michael R. Shapiro, Benjamin Elisabeth, Smela
Copyright Year	2009
Abstract	A novel approach was developed for mapping the location of target DNA in tissue sections. The method combines a high-density, multi-well plate with an innovative single-tube procedure to directly extract, amplify, and detect the DNA in parallel while maintaining the two-dimensional (2D) architecture of the tissue. A 2D map of the gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created from a tissue section and shown to correlate with the spatial area of the sample. It is anticipated that this approach may be easily adapted to assess the status of multiple genes within tissue sections, yielding a molecular map that directly correlates with the histology of the sample. This will provide investigators with a new tool to interrogate the molecular heterogeneity of tissue specimens.
Related Links	http://dx.doi.org/10.1039/b910807f
Ending Page	3534
Page Count	9
Starting Page	3526
File Format	PDF
ISSN	14730197
e-ISSN	14730189
Journal	Lab on a chip
Issue Number	24
Volume Number	9
Language	English
Publisher Date	2009-01-01
Access Restriction	Open
Subject Keyword	Biochemistry Bioengineering Chemistry(all) Biomedical Engineering Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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2D-PCR: a method of mapping DNA in tissue sections†