NDLI: Differential effects of human and plant N-acetylglucosaminyltransferase I (GnTI) in plants

Content Provider	PubMed Central
Author	Maurice, Henquet Bas, Heinhuis Borst, Jan Willem Jochem, Eigenhuijsen Schreuder, Mariëlle Bosch, Dirk Krol, Alexander Van Der
Abstract	In plants and animals, the first step in complex type N-glycan formation on glycoproteins is catalyzed by N-acetylglucosaminyltransferase I (GnTI). We show that the cgl1-1 mutant of Arabidopsis, which lacks GnTI activity, is fully complemented by YFP-labeled plant AtGnTI, but only partially complemented by YFP-labeled human HuGnTI and that this is due to post-transcriptional events. In contrast to AtGnTI-YFP, only low levels of HuGnTI-YFP protein was detected in transgenic plants. In protoplast co-transfection experiments all GnTI-YFP fusion proteins co-localized with a Golgi marker protein, but only limited co-localization of AtGnTI and HuGnTI in the same plant protoplast. The partial alternative targeting of HuGnTI in plant protoplasts was alleviated by exchanging the membrane-anchor domain with that of AtGnTI, but in stably transformed cgl1-1 plants this chimeric GnTI still did not lead to full complementation of the cgl1-1 phenotype. Combined, the results indicate that activity of HuGnTI in plants is limited by a combination of reduced protein stability, alternative protein targeting and possibly to some extend to lower enzymatic performance of the catalytic domain in the plant biochemical environment.
Related Links	http://dx.doi.org/10.1007/s11248-009-9331-7
Ending Page	547
Page Count	13
Starting Page	535
File Format	PDF
ISSN	09628819
e-ISSN	15739368
Journal	Transgenic Research
Issue Number	4
Volume Number	19
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-08-01
Access Restriction	Open
Rights Holder	Springer Netherlands
Subject Keyword	Biotechnology Agronomy and Crop Science Animal Science and Zoology Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Animal Science and Zoology Biotechnology Agronomy and Crop Science

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