NDLI: Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Please wait, while we are loading the content...

Affinity Maturation of an Anti-V Antigen IgG Expressed In Situ Via Adenovirus Gene Delivery Confers Enhanced Protection Against Yersinia pestis Challenge

VEGF Blockade Decreases Tumor Uptake of Systemic Oncolytic Herpes Virus but Enhances Therapeutic Efficacy When Given After Virotherapy

Year: 2010, Volume: 17, Issue: 6

Year: 2010, Volume: 17, Issue: 5

Year: 2010, Volume: 17, Issue: 4

Year: 2010, Volume: 17, Issue: 3

Year: 2010, Volume: 17, Issue: 2

Year: 2010, Volume: 17, Issue: 1

Year: 2009, Volume: 16

Year: 2008, Volume: 15

Year: 2007, Volume: 14

Year: 2006, Volume: 13

Year: 2005, Volume: 12

Year: 2004, Volume: 11

Year: 2003, Volume: 10

Year: 2002, Volume: 9

Year: 2000, Volume: 7

Year: 1999, Volume: 6

Year: 1998, Volume: 5

Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Content Provider	PubMed Central
Author	Chen, Iy Gheysens, O. Ray, S. Wang, Q. Padmanabhan, P. Paulmurugan, R. Loening, Am Rodriguez-porcel, M. Willmann, Jk Sheikh, Ay Nielsen, Ch Hoyt, G. Contag, Ch Robbins, Rc Biswal, S. Wu, Jc Gambhir, Ss
Copyright Year	2010
Abstract	Transcriptional targeting for cardiac gene therapy is limited by the relatively weak activity of most cardiac-specific promoters. We have developed a bidirectional plasmid vector, which uses a two-step transcriptional amplification (TSTA) strategy to enhance the expression of two optical reporter genes, firefly luciferase (fluc) and Renilla luciferase (hrluc), driven by the cardiac troponin T (cTnT) promoter. The vector was characterized in vitro and in living mice using luminometry and bioluminescence imaging to assess its ability to mediate strong, correlated reporter gene expression in a cardiac cell line and the myocardium, while minimizing expression in non-cardiac cell lines and the liver. In vitro, the TSTA system significantly enhanced cTnT-mediated reporter gene expression with moderate preservation of cardiac specificity. After intramyocardial and hydrodynamic tail vein delivery of an hrluc-enhanced variant of the vector, long-term fluc expression was observed in the heart, but not in the liver. In both the cardiac cell line and the myocardium, fluc expression correlated well with hrluc expression. These results show the vector’s ability to effectively amplify and couple transgene expression in a cardiac-specific manner. Further replacement of either reporter gene with a therapeutic gene should allow non-invasive imaging of targeted gene therapy in living subjects.
Related Links	http://dx.doi.org/10.1038/gt.2010.30
Ending Page	838
Page Count	12
Starting Page	827
File Format	PDF
ISSN	09697128
e-ISSN	14765462
Journal	Gene therapy
Issue Number	7
Volume Number	17
Language	English
Publisher Date	2010-07-01
Access Restriction	Open
Subject Keyword	Molecular Medicine Genetics Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

Non-invasive imaging of a transgenic mouse model using a prostate-specific two-step transcriptional amplification strategy

Noninvasive Imaging of Therapeutic Gene Expression Using a Bi-directional Transcriptional Amplification Strategy

Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters

Highly specific transgene expression mediated by a complex adenovirus vector incorporating a prostate-specific amplification feedback loop

Novel Bidirectional Vector Strategy for Amplification of Therapeutic and Reporter Gene Expression

Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain.

Specific Targeting of Gene Therapy to Prostate Cancer Using a Two-step Transcriptional Amplification System

Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Similar Documents

Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain

Non-invasive imaging of a transgenic mouse model using a prostate-specific two-step transcriptional amplification strategy

Noninvasive Imaging of Therapeutic Gene Expression Using a Bi-directional Transcriptional Amplification Strategy

Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters

Highly specific transgene expression mediated by a complex adenovirus vector incorporating a prostate-specific amplification feedback loop

Novel Bidirectional Vector Strategy for Amplification of Therapeutic and Reporter Gene Expression

Enhancement of cell-specific transgene expression from a Tet-Off regulatory system using a transcriptional amplification strategy in the rat brain.

Specific Targeting of Gene Therapy to Prostate Cancer Using a Two-step Transcriptional Amplification System

Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy