NDLI: Autologous Extracellular Cytochrome c Is an Endogenous Ligand for Leucine-rich α2-Glycoprotein and β-Type Phospholipase A2 Inhibitor

Content Provider	PubMed Central
Author	Shirai, Ryoichi 白井, 僚一 Gotou, Rikiya 後藤, 力哉 Hirano, Fumiyasu 平野, 文康 Ikeda, Kiyoshi 池田, 潔 Inoue, Seiji 井上, 晴嗣
Abstract	β-Type phospholipase A2 inhibitory protein (PLIβ) from the serum of the venomous snake Gloydius brevicaudus neutralizes basic phospholipase A2 (PLA2) from its own venom, and it has 33% sequence homology with human leucine-rich α2-glycoprotein (LRG), which has been recently reported to bind cytochrome c (Cyt c) (Cummings, C., Walder, J., Treeful, A., and Jemmerson, R. (2006) Apoptosis 11, 1121–1129). In the present study, PLIβ was found to bind Cyt c. The interactions of LRG and PLIβ with Cyt c were compared by surface plasmon resonance analysis. Human LRG bound horse and snake Cyt c with dissociation constants of 1.58 × 10−13 m and 1.65 × 10−10 m, respectively, but did not bind yeast Cyt c, while G. brevicaudus PLIβ bound horse, snake, and yeast Cyt c with dissociation constants of 1.05 × 10−10 m, 2.37 × 10−12 m, and 1.67 × 10−6 m, respectively. On the other hand, LRG did not show any PLA2 inhibitory activity and did not bind G. brevicaudus basic PLA2, whereas PLIβ bound the basic PLA2 with a dissociation constant of 1.21 × 10−9 m, which is smaller than those with the Cyt c described above. The PLA2 inhibitory activity of PLIβ was also found to be suppressed by the binding of Cyt c to PLIβ. These results suggest that autologous Cyt c is an endogeneous ligand for LRG and PLIβ and that these serum proteins neutralize the autologous Cyt c released from the dead cells.
Related Links	http://dx.doi.org/10.1074/jbc.m110.122788
Ending Page	21614
Page Count	8
Starting Page	21607
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	28
Volume Number	285
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2010-07-09
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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