NDLI: Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S.

Please wait, while we are loading the content...

Year: 2016, Volume: 90

Year: 2015, Volume: 90

Year: 2015, Volume: 89

Year: 2014, Volume: 89

Year: 2014, Volume: 88

Year: 2013, Volume: 87

Year: 2012, Volume: 86

Year: 2011, Volume: 85

Year: 2010, Volume: 84

Year: 2009, Volume: 83

Year: 2008, Volume: 82

Year: 2007, Volume: 81

Year: 2006, Volume: 80

Year: 2005, Volume: 79

Year: 2004, Volume: 78

Year: 2003, Volume: 77

Year: 2002, Volume: 76

Year: 2001, Volume: 75

Year: 2000, Volume: 74

Year: 1999, Volume: 73

Year: 1998, Volume: 72

Year: 1997, Volume: 71

Year: 1996, Volume: 70

Year: 1995, Volume: 69

Year: 1994, Volume: 68

Year: 1993, Volume: 67

Year: 1992, Volume: 66

Year: 1992, Volume: 66, Issue: 12

Year: 1992, Volume: 66, Issue: 11

Year: 1992, Volume: 66, Issue: 10

Year: 1992, Volume: 66, Issue: 9

Year: 1992, Volume: 66, Issue: 8

Year: 1992, Volume: 66, Issue: 7

Year: 1992, Volume: 66, Issue: 6

Year: 1992, Volume: 66, Issue: 5

Year: 1992, Volume: 66, Issue: 4

Protective immune response against foot-and-mouth disease.

Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination.

trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.

Gag proteins of the highly replicative MN strain of human immunodeficiency virus type 1: posttranslational modifications, proteolytic processings, and complete amino acid sequences.

Mutations in the SH3 domain of the src oncogene which decrease association of phosphatidylinositol 3'-kinase activity with pp60v-src and alter cellular morphology.

Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization.

Induced expression of the endogenous beta interferon gene in adenovirus type 5-transformed rat fibroblasts.

Subacute sclerosing panencephalitis virus dominantly interferes with replication of wild-type measles virus in a mixed infection: implication for viral persistence.

BHRF1 of Epstein-Barr virus, which is homologous to human proto-oncogene bcl2, is not essential for transformation of B cells or for virus replication in vitro.

The Hantaan virus M-segment glycoproteins G1 and G2 can be expressed independently.

Expression of intracisternal A-particle-related retroviral element-encoded envelope proteins detected in cell lines.

The sequence context of the initiation codon in the encephalomyocarditis virus leader modulates efficiency of internal translation initiation.

Spacing is crucial for coordination of domain functions within the simian virus 40 core origin of replication.

The vaccinia virus K3L gene product potentiates translation by inhibiting double-stranded-RNA-activated protein kinase and phosphorylation of the alpha subunit of eukaryotic initiation factor 2.

A single base deletion in the 5' noncoding region of Theiler's virus attenuates neurovirulence.

CArG, CCAAT, and CCAAT-like protein binding sites in avian retrovirus long terminal repeat enhancers.

Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol.

Gamma interferon-dependent clearance of cytomegalovirus infection in salivary glands.

Inhibition of poliovirus RNA synthesis by brefeldin A.

v-Src enhances phosphorylation at Ser-282 of the Rous sarcoma virus integrase.

Inhibition of human immunodeficiency virus type 1 Tat activity by coexpression of heterologous trans activators.

Human antibodies recognize multiple distinct type-specific and cross-reactive regions of the minor capsid proteins of human papillomavirus types 6 and 11.

Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S.

Functional comparison of the basic domains of the Tat proteins of human immunodeficiency virus types 1 and 2 in trans activation.

Class I major histocompatibility proteins are an essential component of the simian virus 40 receptor.

Stimulation of lymphokines in Jurkat cells persistently infected with vaccinia virus.

Activation of the human immunodeficiency virus type 1 long terminal repeat by vaccinia virus.

Molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind.

Complex determinants of macrophage tropism in env of simian immunodeficiency virus.

An orthopoxvirus serpinlike gene controls the ability of infected cells to fuse.

High-level expression of the tick-borne encephalitis virus NS1 protein by using an adenovirus-based vector: protection elicited in a murine model.

Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent.

Human cytotoxic T cells stimulated by antigen on dendritic cells recognize the N, SH, F, M, 22K, and 1b proteins of respiratory syncytial virus.

Effects of hydrostatic pressure on a membrane-enveloped virus: high immunogenicity of the pressure-inactivated virus.

Random mutagenesis of the thymidine kinase gene of varicella-zoster virus.

The E6 and E7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells.

Three-dimensional structure of single-shelled bluetongue virus.

Quantitation of a lentivirus in its natural host: simian immunodeficiency virus in African green monkeys.

Rapid in vivo reactivation of herpes simplex virus in latently infected murine ganglionic neurons after transient hyperthermia.

Herpes simplex virus type 1 latency-associated transcription unit promotes anatomical site-dependent establishment and reactivation from latency.

Novel human endogenous sequences related to human immunodeficiency virus type 1.

Reovirus polypeptide sigma 3 and N-terminal myristoylation of polypeptide mu 1 are required for site-specific cleavage to mu 1C in transfected cells.

Cross-reactive and serotype-specific antibodies against foot-and-mouth disease virus generated by different regions of the same synthetic peptide.

A deletion in the simian virus 40 large T antigen impairs lytic replication in monkey cells in vivo but enhances DNA replication in vitro: new complementation function of T antigen.

Antigenic structure of human hepatitis A virus defined by analysis of escape mutants selected against murine monoclonal antibodies.

Induction of interleukin-1 and tumor necrosis factor alpha in brain cultures by human immunodeficiency virus type 1.

Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.

A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH.

VPg gene amplification correlates with infective particle formation in foot-and-mouth disease virus.

Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.

Differentiation-dependent human immunodeficiency virus long terminal repeat regulatory elements active in human teratocarcinoma cells.

Vaccinia virus-specific human CD4+ cytotoxic T-lymphocyte clones.

Detection of receptor-specific murine leukemia virus binding to cells by immunofluorescence analysis.

Low degree of human T-cell leukemia/lymphoma virus type I genetic drift in vivo as a means of monitoring viral transmission and movement of ancient human populations.

Human immunodeficiency virus type 1 glycoprotein precursor retains a CD4-p56lck complex in the endoplasmic reticulum.

Overexpression of the E1B 55-kilodalton (482R) protein of human adenovirus type 12 appears to permit efficient transformation of primary baby rat kidney cells in the absence of the E1B 19-kilodalton protein.

Assembly of hepatitis delta virus particles.

Glycoprotein gI of pseudorabies virus promotes cell fusion and virus spread via direct cell-to-cell transmission.

Replication of nodamura virus after transfection of viral RNA into mammalian cells in culture.

Cellular expression of a functional nodavirus RNA replicon from vaccinia virus vectors.

The bovine papillomavirus constitutive enhancer is essential for viral transformation, DNA replication, and the maintenance of latency.

Both substrate and target oligonucleotide sequences affect in vitro integration mediated by human immunodeficiency virus type 1 integrase protein produced in Saccharomyces cerevisiae.

Role of the apical stem in maintaining the structure and function of adenovirus virus-associated RNA.

Homologous recombination of copackaged retrovirus RNAs during reverse transcription.

Susceptibility of inbred strains of mice to murine AIDS (MAIDS) correlates with target cell expansion and high expression of defective MAIDS virus.

An AP-1 binding site is the predominant cis-acting regulatory element in the 1.2-kilobase early RNA promoter of human cytomegalovirus.

Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein.

Efficacy of oral administration of live herpes simplex virus type 1 as a vaccine.

Reversion of Q beta RNA phage mutants by homologous RNA recombination.

Studies on the fusion peptide of a paramyxovirus fusion glycoprotein: roles of conserved residues in cell fusion.

Multiple isolates and characteristics of human T-cell leukemia virus type II.

Interaction between retroviral U5 RNA and the T psi C loop of the tRNA(Trp) primer is required for efficient initiation of reverse transcription.

Influence of carbohydrate moieties on the immunogenicity of human immunodeficiency virus type 1 recombinant gp160.

Characterization of dexamethasone-induced reactivation of latent bovine herpesvirus 1.

Heterogeneity of the mutation rates of influenza A viruses: isolation of mutator mutants.

Transformation by hamster polyomavirus: identification and functional analysis of the early genes.

Expression of herpes simplex virus type 1 glycoprotein I in baculovirus: preliminary biochemical characterization and protection studies.

Inhibition of human immunodeficiency virus type 1 Rev function by a Rev mutant which interferes with nuclear/nucleolar localization of Rev.

Probing protein-DNA interactions at the long terminal repeat of human immunodeficiency virus type 1 by in vivo footprinting.

Mechanism of interferon action: alpha and gamma interferons differentially affect mRNA levels of the catalytic subunit of protein kinase A and protein Mx in human cells.

A chimeric poliovirus/CD4 receptor confers susceptibility to poliovirus on mouse cells.

Diversity of T-cell receptors in virus-specific cytotoxic T lymphocytes recognizing three distinct viral epitopes restricted by a single major histocompatibility complex molecule.

Generation of "natural killer cell-escape" variants of Pichinde virus during acute and persistent infections.

Autopresentation of hepatitis B virus envelope antigens by T cells.

A cluster of dispensable genes within the human cytomegalovirus genome short component: IRS1, US1 through US5, and the US6 family.

Human immunodeficiency virus type 1 entry into T cells: more-rapid escape from an anti-V3 loop than from an antireceptor antibody.

Simian virus 40 small t antigen trans activates the adenovirus E2A promoter by using mechanisms distinct from those used by adenovirus E1A.

Evolutionary insights on the origin of human T-cell lymphoma/leukemia virus type I (HTLV-I) derived from sequence analysis of a new HTLV-I variant from Papua New Guinea.

Human and mouse Mx proteins inhibit different steps of the influenza virus multiplication cycle.

Human immunodeficiency virus type 1 major neutralizing determinant exposed on hepatitis B surface antigen particles is highly immunogenic in primates.

Macrophage tropism determinants of human immunodeficiency virus type 1 in vivo.

Definition of the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex protein activation domain by functional exchange.

Human immunodeficiency virus type 1 tropism for brain microglial cells is determined by a region of the env glycoprotein that also controls macrophage tropism.

Mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells.

Year: 1992, Volume: 66, Issue: 3

Year: 1992, Volume: 66, Issue: 2

Year: 1992, Volume: 66, Issue: 1

Year: 1991, Volume: 65

Year: 1990, Volume: 64

Year: 1989, Volume: 63

Year: 1988, Volume: 62

Year: 1987, Volume: 61

Year: 1986, Volume: 60

Year: 1986, Volume: 59

Year: 1986, Volume: 58

Year: 1986, Volume: 57

Year: 1985, Volume: 56

Year: 1985, Volume: 55

Year: 1985, Volume: 54

Year: 1985, Volume: 53

Year: 1984, Volume: 52

Year: 1984, Volume: 51

Year: 1984, Volume: 50

Year: 1984, Volume: 49

Year: 1983, Volume: 48

Year: 1983, Volume: 47

Year: 1983, Volume: 46

Year: 1983, Volume: 45

Year: 1982, Volume: 44

Year: 1982, Volume: 43

Year: 1982, Volume: 42

Year: 1982, Volume: 41

Year: 1981, Volume: 40

Year: 1981, Volume: 39

Year: 1981, Volume: 38

Year: 1981, Volume: 37

Year: 1980, Volume: 36

Year: 1980, Volume: 35

Year: 1980, Volume: 34

Year: 1980, Volume: 33

Year: 1979, Volume: 32

Year: 1979, Volume: 31

Year: 1979, Volume: 30

Year: 1979, Volume: 29

Year: 1978, Volume: 28

Year: 1978, Volume: 27

Year: 1978, Volume: 26

Year: 1978, Volume: 25

Year: 1977, Volume: 24

Year: 1977, Volume: 23

Year: 1977, Volume: 22

Year: 1977, Volume: 21

Year: 1976, Volume: 20

Year: 1976, Volume: 19

Year: 1976, Volume: 18

Year: 1976, Volume: 17

Year: 1975, Volume: 16

Year: 1975, Volume: 15

Year: 1974, Volume: 14

Year: 1974, Volume: 13

Year: 1973, Volume: 12

Year: 1973, Volume: 11

Year: 1972, Volume: 10

Year: 1972, Volume: 9

Year: 1971, Volume: 8

Year: 1971, Volume: 7

Year: 1970, Volume: 6

Year: 1970, Volume: 5

Year: 1969, Volume: 4

Year: 1969, Volume: 3

Year: 1968, Volume: 2

Year: 1967, Volume: 1

Immortalization of primary epithelial cells requires first- and second-exon functions of adenovirus type 5 12S.

Content Provider	PubMed Central
Author	Quinlan, M. P. Douglas, J. L.
Abstract	Immortalization of primary cells is a multistep process. The adenovirus E1A 12S gene product is a member of the class of oncoproteins that have the ability to establish primary cells as cell lines in culture. It is encoded by two exons. Extensive mutational analysis demonstrates that four regions of the E1A 12S gene, encoded by both exons, are necessary for immortalization of primary epithelial cells. Expression of two regions is necessary to activate quiescent cells into the cell cycle but is unable to extend the life span of these cells in culture and thus cannot immortalize them. These regions are encoded by the first exon. A third first-exon region, for which no function has yet been identified, is also required. These three regions are also required for 12S to cooperate with an activated ras gene to bring about tumorigenic transformation. The fourth region is required to maintain the cells in a proliferative mode, extend their life span in culture, and induce an autocrine growth factor. These functions are encoded by the second exon. The cells immortalized by wild-type 12S and immortalization-competent mutants retain their epithelial morphology and expression of keratin and vimentin intermediate filament proteins.
Starting Page	2020
File Format	PDF
ISSN	10985514
e-ISSN	10985514
Journal	Journal of Virology
Issue Number	4
Volume Number	66
Language	English
Publisher Date	1992-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Virology Immunology Microbiology Insect Science

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in