NDLI: Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine

Content Provider	PubMed Central
Author	Tokuhara, Daisuke Yuki, Yoshikazu Nochi, Tomonori Kodama, Toshio Mio, Mejima Kurokawa, Shiho Takahashi, Yuko Nanno, Masanobu Nakanishi, Ushio Takaiwa, Fumio Honda, Takeshi Kiyono, Hiroshi
Abstract	Cholera and enterotoxigenic Escherichia coli (ETEC) are among the most common causes of acute infantile gastroenteritis globally. We previously developed a rice-based vaccine that expressed cholera toxin B subunit (MucoRice-CTB) and had the advantages of being cold chain–free and providing protection against cholera toxin (CT)–induced diarrhea. To advance the development of MucoRice-CTB for human clinical application, we investigated whether the CTB-specific secretory IgA (SIgA) induced by MucoRice-CTB gives longstanding protection against diarrhea induced by Vibrio cholerae and heat-labile enterotoxin (LT)–producing ETEC (LT-ETEC) in mice. Oral immunization with MucoRice-CTB stored at room temperature for more than 3 y provided effective SIgA-mediated protection against CT- or LT-induced diarrhea, but the protection was impaired in polymeric Ig receptor–deficient mice lacking SIgA. The vaccine gave longstanding protection against CT- or LT-induced diarrhea (for ≥6 months after primary immunization), and a single booster immunization extended the duration of protective immunity by at least 4 months. Furthermore, MucoRice-CTB vaccination prevented diarrhea in the event of V. cholerae and LT-ETEC challenges. Thus, MucoRice-CTB is an effective long-term cold chain–free oral vaccine that induces CTB-specific SIgA-mediated longstanding protection against V. cholerae– or LT-ETEC–induced diarrhea.
Related Links	http://dx.doi.org/10.1073/pnas.0914121107
Ending Page	8799
Page Count	6
Starting Page	8794
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	107
Language	English
Publisher	National Academy of Sciences
Publisher Date	2010-05-11
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Identification of a protein secretory pathway for the secretion of heat-labile enterotoxin by an enterotoxigenic strain of Escherichia coli

Detection by a staphylococcal coagglutination test of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli.

Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.

Designing Vaccines to Neutralize Effective Toxin Delivery by Enterotoxigenic Escherichia coli

Confronting Challenges to Enterotoxigenic Escherichia coli Vaccine Development

Detection of heat-labile-enterotoxin-producing colonies of Escherichia coli and Vibrio cholerae by solid-phase sandwich radioimmunoassays.

Immunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli.

Factors Affecting Release of Heat-Labile Enterotoxin by Enterotoxigenic Escherichia coli

Development of a vaccine of cross-linked heat-stable and heat-labile enterotoxins that protects against Escherichia coli producing either enterotoxin.

Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine

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Identification of a protein secretory pathway for the secretion of heat-labile enterotoxin by an enterotoxigenic strain of Escherichia coli

Detection by a staphylococcal coagglutination test of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli.

Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.

Designing Vaccines to Neutralize Effective Toxin Delivery by Enterotoxigenic Escherichia coli

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Detection of heat-labile-enterotoxin-producing colonies of Escherichia coli and Vibrio cholerae by solid-phase sandwich radioimmunoassays.

Immunization of rats with heat-labile enterotoxin provides uniform protection against heterologous serotypes of enterotoxigenic Escherichia coli.

Factors Affecting Release of Heat-Labile Enterotoxin by Enterotoxigenic Escherichia coli

Development of a vaccine of cross-linked heat-stable and heat-labile enterotoxins that protects against Escherichia coli producing either enterotoxin.

Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine