NDLI: Characterization of the Proximal Ligand in the P420 Form of iNOS

Content Provider	PubMed Central
Author	Sabat, Joseph Stuehr, Dennis J. Yeh, Syun-ru Rousseau, Denis L.
Abstract	The nitric oxide (NO) produced by inducible Nitric Oxide Synthase (iNOS) up-regulates the expression of heme oxygenase (HO), which in turn produces carbon monoxide (CO) that down-regulates iNOS activity by reducing its expression level or by inhibiting its activity by converting it to an inactive P420 form (iNOSP420). Accordingly, CO has been considered as a potentially important attenuator of inflammation. Despite its importance, the nature of the proximal heme ligand of the iNOSP420 species remains elusive. Here we show that the 221 cm−1 mode of the photoproduct of iNOSP420 does not exhibit any H2O-D2O solvent isotope shift such as that found in the iron-histidine stretching mode of myoglobin, indicating that the proximal ligand of iNOSP420 is not a histidine. The νFe-CO and νC-O data reveal that the proximal heme ligand of iNOSP420 is consistent with a protonated thiol, instead of a thiolate anion. Furthermore, the optical absorption properties of iNOSP420 are similar to those of a neutral thiol-heme model complex, but not myoglobin. Together the data support the scenario that iNOSP420 is inactivated by protonation of the native proximal thiolate ligand to a neutral thiol, instead of by ligand switching to a histidine, as prior studies have suggested.
Related Links	http://dx.doi.org/10.1021/ja901016a
Ending Page	12192
Page Count	7
Starting Page	12186
File Format	PDF
ISSN	00027863
e-ISSN	15205126
Journal	Journal of the American Chemical Society
Issue Number	34
Volume Number	131
Language	English
Publisher Date	2009-09-02
Access Restriction	Open
Subject Keyword	Colloid and Surface Chemistry Biochemistry Chemistry(all) Catalysis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Colloid and Surface Chemistry Biochemistry Catalysis

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Proximal Ligand Electron Donation and Reactivity of the Cytochrome P450 Ferric-Peroxo Anion

Spectroscopic Characterization of YedY: The Role of Sulfur Coordination in a Mo(V) Sulfite Oxidase Family Enzyme Form

Direct and Quantitative Characterization of Dynamic Ligand Exchange between Coordination-Driven Self-Assembled Supramolecular Polygons

Ligand-Controlled,Tunable Silver-Catalyzed C–HAmination

ComprehensiveFe–Ligand Vibration Identificationin {FeNO}6 Hemes

Ligand-Dependent Activation and Deactivation of the Human Adenosine A2A Receptor

Ligand–ReceptorInteraction Catalyzes the Aggregation of Small Molecules To InduceCell Necroptosis

Solution Structure of a β-Peptide Ligand for hDM2

Ultrafast dynamics of diatomic ligand binding to nitrophorin 4

Characterization of the Proximal Ligand in the P420 Form of iNOS

Similar Documents

Proximal Ligand Electron Donation and Reactivity of the Cytochrome P450 Ferric-Peroxo Anion

Spectroscopic Characterization of YedY: The Role of Sulfur Coordination in a Mo(V) Sulfite Oxidase Family Enzyme Form

Direct and Quantitative Characterization of Dynamic Ligand Exchange between Coordination-Driven Self-Assembled Supramolecular Polygons

Ligand-Controlled,Tunable Silver-Catalyzed C–HAmination

ComprehensiveFe–Ligand Vibration Identificationin {FeNO}6 Hemes

Ligand-Dependent Activation and Deactivation of the Human Adenosine A2A Receptor

Ligand–ReceptorInteraction Catalyzes the Aggregation of Small Molecules To InduceCell Necroptosis

Solution Structure of a β-Peptide Ligand for hDM2

Ultrafast dynamics of diatomic ligand binding to nitrophorin 4

Characterization of the Proximal Ligand in the P420 Form of iNOS