NDLI: The Legionella pneumophila LetA/LetS Two-Component System Exhibits Rheostat-Like Behavior▿ †

Content Provider	PubMed Central
Author	Edwards, Rachel L. Jules, Matthieu Sahr, Tobias Carmen, Buchrieser Swanson, Michele S.
Copyright Year	2010
Abstract	When confronted with metabolic stress, replicative Legionella pneumophila bacteria convert to resilient, infectious cells equipped for transmission. Differentiation is promoted by the LetA/LetS two-component system, which belongs to a family of signal-transducing proteins that employ a four-step phosphorelay to regulate gene expression. Histidine 307 of LetS was essential to switch on the transmission profile, but a threonine substitution at position 311 (T311M) suggested a rheostat-like function. The letS(T311M) bacteria resembled the wild type (WT) for some traits and letS null mutants for others, whereas they displayed intermediate levels of infectivity, cytotoxicity, and lysosome evasion. Although only 30 to 50% of letS(T311M) mutants became motile, flow cytometry determined that every cell eventually activated the flagellin promoter to WT levels, but expression was delayed. Likewise, letS(T311M) mutants exhibited delayed induction of RsmY and RsmZ, regulatory RNAs that relieve CsrA repression of transmission traits. Transcriptional profile analysis revealed that letS(T311M) mutants expressed the flagellar regulon and multiple other transmissive-phase loci at a higher cell density than the WT. Accordingly, we postulate that the letS(T311M) mutant may relay phosphate less efficiently than the WT LetS sensor protein, leading to sluggish gene expression and a variety of phenotypic profiles. Thus, as first described for BvgA/BvgS, rather than acting as on/off switches, this family of two-component systems exhibit rheostat activity that likely confers versatility as microbes adapt to fluctuating environments.
Related Links	http://dx.doi.org/10.1128/iai.01107-09
Ending Page	2583
Page Count	13
Starting Page	2571
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	6
Volume Number	78
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2010-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The LetE Protein Enhances Expression of Multiple LetA/LetS-Dependent Transmission Traits by Legionella pneumophila

The PmrA/PmrB Two-Component System of Legionella pneumophila Is a Global Regulator Required for Intracellular Replication within Macrophages and Protozoa▿ †

Isolation of Plasmids in Legionella pneumophila and Legionella-Like Organisms

Isolation of plasmids in Legionella pneumophila and Legionella-like organisms.

Specificity of Legionella pneumophila and Coxiella burnetii Vacuoles and Versatility of Legionella pneumophila Revealed by Coinfection

Absence of siderophore-like activity in Legionella pneumophila supernatants.

Antibody-independent binding of complement component C1q by Legionella pneumophila.

Legionella pneumophila Pathogenesis in the Galleria mellonella Infection Model

Legionella pneumophila Replication Vacuole Formation Involves Rapid Recruitment of Proteins of the Early Secretory System

The Legionella pneumophila LetA/LetS Two-Component System Exhibits Rheostat-Like Behavior▿ †

Similar Documents

The LetE Protein Enhances Expression of Multiple LetA/LetS-Dependent Transmission Traits by Legionella pneumophila

The PmrA/PmrB Two-Component System of Legionella pneumophila Is a Global Regulator Required for Intracellular Replication within Macrophages and Protozoa▿ †

Isolation of Plasmids in Legionella pneumophila and Legionella-Like Organisms

Isolation of plasmids in Legionella pneumophila and Legionella-like organisms.

Specificity of Legionella pneumophila and Coxiella burnetii Vacuoles and Versatility of Legionella pneumophila Revealed by Coinfection

Absence of siderophore-like activity in Legionella pneumophila supernatants.

Antibody-independent binding of complement component C1q by Legionella pneumophila.

Legionella pneumophila Pathogenesis in the Galleria mellonella Infection Model

Legionella pneumophila Replication Vacuole Formation Involves Rapid Recruitment of Proteins of the Early Secretory System

The Legionella pneumophila LetA/LetS Two-Component System Exhibits Rheostat-Like Behavior▿ †