NDLI: Identification of Chlamydia trachomatis Outer Membrane Complex Proteins by Differential Proteomics▿ †

Content Provider	PubMed Central
Author	Liu, Xiaoyun Mary, Afrane Clemmer, David E. Zhong, Guangming Nelson, David E.
Copyright Year	2010
Abstract	The extracellular chlamydial infectious particle, or elementary body (EB), is enveloped by an intra- and intermolecular cysteine cross-linked protein shell called the chlamydial outer membrane complex (COMC). A few abundant proteins, including the major outer membrane protein and cysteine-rich proteins (OmcA and OmcB), constitute the overwhelming majority of COMC proteins. The identification of less-abundant COMC proteins has been complicated by limitations of proteomic methodologies and the contamination of COMC fractions with abundant EB proteins. Here, we used parallel liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analyses of Chlamydia trachomatis serovar L2 434/Bu EB, COMC, and Sarkosyl-soluble EB fractions to identify proteins enriched or depleted from COMC. All well-described COMC proteins were specifically enriched in the COMC fraction. In contrast, multiple COMC-associated proteins found in previous studies were strongly enriched in the Sarkosyl-soluble fraction, suggesting that these proteins are not COMC components or are not stably associated with COMC. Importantly, we also identified novel proteins enriched in COMC. The list of COMC proteins identified in this study has provided reliable information for further understanding chlamydial protein secretion systems and modeling COMC and EB structures.
Related Links	http://dx.doi.org/10.1128/JB.01628-09
Starting Page	2852
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	11
Volume Number	192
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2010-06-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Chlamydia trachomatis Outer Membrane Complex Protein B (OmcB) Is Processed by the Protease CPAF

Characterization of Outer Membrane Proteins in Chlamydia trachomatis LGV Serovar L2

Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatis.

Diversity of Chlamydia trachomatis major outer membrane protein genes.

Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2.

Topological Analysis of Chlamydia trachomatis L2 Outer Membrane Protein 2

Structural and Functional Analyses of the Major Outer Membrane Protein of Chlamydia trachomatis ▿

Functional analysis of the major outer membrane protein gene promoters of Chlamydia trachomatis.

Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.

Identification of Chlamydia trachomatis Outer Membrane Complex Proteins by Differential Proteomics▿ †

Similar Documents

Chlamydia trachomatis Outer Membrane Complex Protein B (OmcB) Is Processed by the Protease CPAF

Characterization of Outer Membrane Proteins in Chlamydia trachomatis LGV Serovar L2

Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatis.

Diversity of Chlamydia trachomatis major outer membrane protein genes.

Identification by sequence analysis of two-site posttranslational processing of the cysteine-rich outer membrane protein 2 of Chlamydia trachomatis serovar L2.

Topological Analysis of Chlamydia trachomatis L2 Outer Membrane Protein 2

Structural and Functional Analyses of the Major Outer Membrane Protein of Chlamydia trachomatis ▿

Functional analysis of the major outer membrane protein gene promoters of Chlamydia trachomatis.

Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.

Identification of Chlamydia trachomatis Outer Membrane Complex Proteins by Differential Proteomics▿ †