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| Content Provider | PubMed Central |
|---|---|
| Author | Song, Kilyoung Lee, Eunsong |
| Copyright Year | 2007 |
| Abstract | This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 µM CYS or 100 µM β-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos. |
| Related Links | http://dx.doi.org/10.4142/jvs.2007.8.1.81 |
| Starting Page | 81 |
| File Format | |
| ISSN | 1976555X |
| e-ISSN | 1976555X |
| Journal | Journal of Veterinary Science |
| Issue Number | 1 |
| Volume Number | 8 |
| Language | English |
| Publisher | The Korean Society of Veterinary Science |
| Publisher Date | 2007-03-01 |
| Access Restriction | Open |
| Rights Holder | The Korean Society of Veterinary Science |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
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