NDLI: Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22.

Please wait, while we are loading the content...

Year: 2016, Volume: 113

Year: 2015, Volume: 112

Year: 2014, Volume: 111

Year: 2013, Volume: 110

Year: 2012, Volume: 109

Year: 2011, Volume: 109

Year: 2011, Volume: 108

Year: 2010, Volume: 107

Year: 2009, Volume: 106

Year: 2009, Volume: 94

Year: 2008, Volume: 105

Year: 2007, Volume: 104

Year: 2006, Volume: 103

Year: 2005, Volume: 103

Year: 2005, Volume: 102

Year: 2004, Volume: 101

Year: 2003, Volume: 100

Year: 2002, Volume: 99

Year: 2001, Volume: 98

Year: 2000, Volume: 97

Year: 1999, Volume: 96

Year: 1998, Volume: 95

Year: 1997, Volume: 94

Year: 1996, Volume: 93

Year: 1995, Volume: 92

Year: 1994, Volume: 91

Year: 1993, Volume: 90

Year: 1992, Volume: 89

Year: 1991, Volume: 88

Year: 1990, Volume: 87

Year: 1989, Volume: 86

Year: 1989, Volume: 86, Issue: Suppl

Year: 1989, Volume: 86, Issue: 24

Year: 1989, Volume: 86, Issue: 23

Year: 1989, Volume: 86, Issue: 22

Year: 1989, Volume: 86, Issue: 21

Year: 1989, Volume: 86, Issue: 20

Year: 1989, Volume: 86, Issue: 19

Year: 1989, Volume: 86, Issue: 18

Year: 1989, Volume: 86, Issue: 17

Year: 1989, Volume: 86, Issue: 16

Year: 1989, Volume: 86, Issue: 15

Year: 1989, Volume: 86, Issue: 14

Year: 1989, Volume: 86, Issue: 13

Year: 1989, Volume: 86, Issue: 12

Year: 1989, Volume: 86, Issue: 11

Year: 1989, Volume: 86, Issue: 10

Year: 1989, Volume: 86, Issue: 9

Year: 1989, Volume: 86, Issue: 8

Year: 1989, Volume: 86, Issue: 7

Transactivation of both early and late simian virus 40 promoters by large tumor antigen does not require nuclear localization of the protein.

Cloning of the mRNA for the protein that crosslinks to the egg peptide speract.

High levels of double-stranded transferred DNA (T-DNA) processing from an intact nopaline Ti plasmid.

Identification of an epitope shared by the DNA-binding domain of glucocorticoid receptor and the B chain of insulin.

Human immunodeficiency virus tat-activated expression of poliovirus protein 2A inhibits mRNA translation.

Oxygen-sensitive ribonucleoside triphosphate reductase is present in anaerobic Escherichia coli.

Identifying determinants of folding and activity for a protein of unknown structure.

Human immunodeficiency viral long terminal repeat is functional and can be trans-activated in Escherichia coli.

DNA bending is induced by a transcription factor that interacts with the human c-FOS and alpha-actin promoters.

Replacement of aspartic acid-96 by asparagine in bacteriorhodopsin slows both the decay of the M intermediate and the associated proton movement.

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

In vitro transcription directed from the somatostatin promoter is dependent upon a purified 43-kDa DNA-binding protein.

Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.

Crystal structure of core streptavidin determined from multiwavelength anomalous diffraction of synchrotron radiation.

Proline isomerism leads to multiple folded conformations of calbindin D9k: direct evidence from two-dimensional 1H NMR spectroscopy.

Perfection of a synaptic receptor: kinetics and energetics of the acetylcholine receptor.

On the origin and transmission of force in actomyosin subfragment 1.

Anthrax toxin: channel-forming activity of protective antigen in planar phospholipid bilayers.

Regulation of heat production in the inflorescences of an Arum lily by endogenous salicylic acid

Mobility of the maize suppressor-mutator element in transgenic tobacco cells.

The ras and myc oncogenes cooperate in tumor induction in many tissues when introduced into midgestation mouse embryos by retroviral vectors.

Transforming growth factor beta reverses the glucocorticoid-induced wound-healing deficit in rats: possible regulation in macrophages by platelet-derived growth factor.

Tyrosine-specific protein phosphorylation is regulated by glycoprotein IIb-IIIa in platelets.

31P nuclear magnetic resonance study of the metabolic pools of adenosine triphosphate in cultured bovine adrenal medullary chromaffin cells.

Signal transduction pathways in the induction of 2',5'-oligoadenylate synthetase gene expression by interferon alpha/beta.

Binding of a soluble factor of Escherichia coli to preproteins does not require ATP and appears to be the first step in protein export.

Phosphorylation and associated translocation of the 87-kDa protein, a major protein kinase C substrate, in isolated nerve terminals.

Deregulated expression of human c-jun transforms primary rat embryo cells in cooperation with an activated c-Ha-ras gene and transforms rat-1a cells as a single gene.

Neonatal induction of a nuclear protein that binds to the c-fos enhancer.

On the evolutionary origin of the plant mitochondrion and its genome

Bilaterians of the Precambrian—Cambrian transition and the annelid—arthropod relationship

Repair of the Escherichia coli chromosome after in vivo scission by the EcoRI endonuclease.

An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation.

Mutagenic specificity of a potent carcinogen, benzo[c]phenanthrene (4R,3S)-dihydrodiol (2S,1R)-epoxide, which reacts with adenine and guanine in DNA.

Exchange of terminal portions of X- and Y-chromosomal short arms in human XY females.

Polycythemia in transgenic mice expressing the human erythropoietin gene.

High frequency of large spontaneous deletions of DNA in tumor-derived CHEF cells.

Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.

Major histocompatibility complex class I-restricted activation of cloned T cells by a soluble protein in the absence of accessory cells.

Comparison of the secondary structures of human class I and class II major histocompatibility complex antigens by Fourier transform infrared and circular dichroism spectroscopy.

Molecular heterogeneity of gamma delta T-cell antigen receptors expressed by CD4- CD8- T-cell clones from normal donors: both disulfide- and non-disulfide-linked receptors are delta TCS1+.

Tumor necrosis factor alpha induces proteins that bind specifically to kappa B-like enhancer elements and regulate interleukin 2 receptor alpha-chain gene expression in primary human T lymphocytes.

Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B.

Molecular basis for expression of the A48 regulatory idiotope on antibodies encoded by immunoglobulin variable-region genes from various families.

Isotype switching by a microinjected mu immunoglobulin heavy chain gene in transgenic mice.

TCR3: a third T-cell receptor in the chicken.

Light-chain gene expression before heavy-chain gene rearrangement in pre-B cells transformed by Epstein-Barr virus.

Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes.

Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone.

Interleukin 1 as an autocrine growth factor for acute myeloid leukemia cells.

Inflammatory mediator-induced hypothalamic-pituitary-adrenal axis activation is defective in streptococcal cell wall arthritis-susceptible Lewis rats.

Muscle coenzyme Q deficiency in familial mitochondrial encephalomyopathy.

Molecular cloning of two west African human immunodeficiency virus type 2 isolates that replicate well in macrophages: a Gambian isolate, from a patient with neurologic acquired immunodeficiency syndrome, and a highly divergent Ghanian isolate.

Ca2+-channel blockers inhibit the action of recombinant platelet-derived growth factor in vascular smooth muscle cells.

Lipoproteins alter the catalytic behavior of the platelet-activating factor acetylhydrolase in human plasma.

Ovarian steroid treatment blocks a postmenopausal increase in blood monocyte interleukin 1 release.

Mutations in the KRAS2 oncogene during progressive stages of human colon carcinoma.

Isolation and characterization of the human parathyroid hormone-like peptide gene.

Molecular basis of adult-onset and chronic GM2 gangliosidoses in patients of Ashkenazi Jewish origin: substitution of serine for glycine at position 269 of the alpha-subunit of beta-hexosaminidase.

Cachectin alters anterior pituitary hormone release by a direct action in vitro.

Colorimetric detection of specific DNA segments amplified by polymerase chain reactions.

Subtelomeric chromosome deletions in field isolates of Plasmodium falciparum and their relationship to loss of cytoadherence in vitro.

Molecular and biological characterization of a replication competent human immunodeficiency type 2 (HIV-2) proviral clone.

Purification of the migration stimulating factor produced by fetal and breast cancer patient fibroblasts.

Selective infection of human CD4+ cells by simian immunodeficiency virus: productive infection associated with envelope glycoprotein-induced fusion.

Molecular cloning of feline immunodeficiency virus.

Microbial glycolipids: possible virulence factors that scavenge oxygen radicals.

Information storage in the nervous system of Aplysia: specific proteins affected by serotonin and cAMP.

Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22.

Regulation of neuronal oxytocin mRNA by ovarian steroids in the mature and developing hypothalamus.

Sodium channels in dendrites of rat cortical pyramidal neurons.

A Drosophila gene encoding a protein resembling the human beta-amyloid protein precursor.

Prostaglandin E2 and its methyl ester reduce cataplexy in canine narcolepsy.

Neural regulation of sex pheromone biosynthesis in Heliothis moths

Striatal phosphoproteins in Parkinson disease and progressive supranuclear palsy.

Muscarinic agonists and phorbol esters increase tyrosine phosphorylation of a 40-kilodalton protein in hippocampal slices.

Steroid hormones: modulators of Na+ absorption and Cl- secretion in cultured tracheal epithelia.

Puberty in monkeys is triggered by chemical stimulation of the hypothalamus.

Activation of single-channel currents in mouse fibroblasts by platelet-derived growth factor.

Endotoxin pretreatment increases endogenous myocardial catalase activity and decreases ischemia-reperfusion injury of isolated rat hearts.

Year: 1989, Volume: 86, Issue: 6

Year: 1989, Volume: 86, Issue: 5

Year: 1989, Volume: 86, Issue: 4

Year: 1989, Volume: 86, Issue: 3

Year: 1989, Volume: 86, Issue: 2

Year: 1989, Volume: 86, Issue: 1

Year: 1988, Volume: 85

Year: 1987, Volume: 84

Year: 1986, Volume: 83

Year: 1985, Volume: 82

Year: 1984, Volume: 81

Year: 1983, Volume: 80

Year: 1982, Volume: 79

Year: 1981, Volume: 78

Year: 1980, Volume: 77

Year: 1979, Volume: 76

Year: 1978, Volume: 75

Year: 1977, Volume: 74

Year: 1976, Volume: 73

Year: 1975, Volume: 72

Year: 1974, Volume: 71

Year: 1973, Volume: 70

Year: 1972, Volume: 69

Year: 1971, Volume: 68

Year: 1970, Volume: 67

Year: 1970, Volume: 66

Year: 1970, Volume: 65

Year: 1969, Volume: 64

Year: 1969, Volume: 63

Year: 1969, Volume: 62

Year: 1968, Volume: 61

Year: 1968, Volume: 60

Year: 1968, Volume: 59

Year: 1967, Volume: 58

Year: 1967, Volume: 57

Year: 1966, Volume: 56

Year: 1966, Volume: 55

Year: 1965, Volume: 54

Year: 1965, Volume: 53

Year: 1964, Volume: 52

Year: 1964, Volume: 51

Year: 1963, Volume: 50

Year: 1963, Volume: 49

Year: 1962, Volume: 48

Year: 1961, Volume: 47

Year: 1960, Volume: 46

Year: 1959, Volume: 45

Year: 1958, Volume: 44

Year: 1957, Volume: 43

Year: 1956, Volume: 42

Year: 1955, Volume: 41

Year: 1954, Volume: 40

Year: 1953, Volume: 39

Year: 1952, Volume: 38

Year: 1951, Volume: 37

Year: 1950, Volume: 36

Year: 1949, Volume: 35

Year: 1948, Volume: 34

Year: 1947, Volume: 33

Year: 1946, Volume: 32

Year: 1945, Volume: 31

Year: 1944, Volume: 30

Year: 1943, Volume: 29

Year: 1942, Volume: 28

Year: 1941, Volume: 27

Year: 1940, Volume: 26

Year: 1939, Volume: 25

Year: 1938, Volume: 24

Year: 1937, Volume: 23

Year: 1936, Volume: 22

Year: 1935, Volume: 21

Year: 1934, Volume: 20

Year: 1933, Volume: 19

Year: 1932, Volume: 18

Year: 1931, Volume: 17

Year: 1930, Volume: 16

Year: 1929, Volume: 15

Year: 1928, Volume: 14

Year: 1927, Volume: 13

Year: 1926, Volume: 12

Year: 1925, Volume: 11

Year: 1924, Volume: 10

Year: 1923, Volume: 9

Year: 1922, Volume: 8

Year: 1921, Volume: 7

Year: 1920, Volume: 6

Year: 1919, Volume: 5

Year: 1918, Volume: 4

Year: 1917, Volume: 3

Year: 1916, Volume: 2

Year: 1915, Volume: 1

Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): developmental and tissue expression in the rat, and mapping of its human homologue to chromosomes 1 and 22.

Content Provider	PubMed Central
Author	Spinner, N. Snyder, S. Anderson, J. Goldgaber, D. Smulowitz, M. Carroll, Z. Emanuel, B. Breitner, J. Rubin, L. Lieberburg, I.
Abstract	Neurofilaments (NFs) are the intermediate filaments specific to nervous tissue. They are probably essential to the tensile strength of the neuron, as well as to transport of molecules and organelles within the axon. Three peptides with apparent molecular masses of approximately 68 (NF-L), 145 (NF-M), and 200 (NF-H) kDa appear to be the major components of NF. The expression of these peptides is specific to nervous tissue and is developmentally regulated. Recently, complete cDNAs encoding NF-L and NF-M, and partial cDNAs encoding NF-H, have been described. To better understand the normal and pathophysiology of NFs we chose to clone the cDNA encoding the rat NF-H peptide. Using monoclonal antibodies that recognized NF-H, we screened a rat brain lambda gt11 library and identified a clone that contained a 2100-nucleotide cDNA insert representing the carboxyl-terminal portion of the NF-H protein. Anti-fusion protein antibodies recognized the NF-H peptide on immunoblots and stained fibrillar structures only in neurons. The cDNA recognized a 4500-nucleotide polyadenylated mRNA that was present only in nervous tissue and a 3500-nucleotide mRNA in adrenal. Brain NF-H mRNA levels were tightly developmentally regulated and paralleled the levels of NF-H peptide on immunoblots. Nuclear runoff studies showed that the 20-fold developmental increase in the NF-H message was due only in part to a 4-fold increase in its transcription rate. Levels of NF-H mRNA varied 20-fold among brain regions, with highest levels in pons/medulla, spinal cord, and cerebellum, and lowest levels in olfactory bulb and hypothalamus. Transcription studies revealed only a 2-fold difference in the transcription rates among these brain regions. Based on these results, we infer that half of the developmental increase and most of the interregional variation in the levels of the NF-H mRNA are mediated through message stabilization. Sequence information revealed that the carboxyl-terminal region of the NF-H peptide contained a unique serine-, proline-, alanine-, glutamic acid-, and lysine-rich repeat. The serine residues are likely sites of phosphorylation in the mature peptide. Genomic blots revealed a single copy of the gene in the rat genome and two copies in the human genome. In situ hybridizations performed on human chromosomes mapped the NF-H gene to chromosomes 1 and 22. Whether one copy is a pseudogene remains to be determined.
Starting Page	2463
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	7
Volume Number	86
Language	English
Publisher Date	1989-04-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in