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| Content Provider | PubMed Central |
|---|---|
| Author | Balkwill, D. L. Casida, L. E. |
| Abstract | The indigenous microflora of soil were released from the soil materials and concentrated without the occurrence of growth by use of a blending-simple centrifugation procedure. The cell concentrate was then frozen-etched and viewed by transmission electron microscopy. Criteria were established for detecting microbial cells among the residual soil debris. The freeze-etching of the soil cell concentrate provided results on cell size distributions in agreement with those obtained by thin sectioning. However, the blending-simple centrifugation procedure for cell release and concentration from soil allowed the observation of large cells (≥1.0 μm in diameter) which apparently are missed by the “exhaustive centrifugal washing” cell separation-concentration procedure. The procedure of blending-simple centrifugation combined with the viewing of frozenetched preparations allowed evaluations of the soil microflora for cellular diameters, length-width ratios, shapes, and structure. |
| Starting Page | 1319 |
| File Format | |
| ISSN | 10985530 |
| e-ISSN | 10985530 |
| Journal | Journal of Bacteriology |
| Issue Number | 3 |
| Volume Number | 114 |
| Language | English |
| Publisher Date | 1973-06-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Biology Microbiology |
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