NDLI: Lipid droplet analysis in caveolin-deficient adipocytes: alterations in surface phospholipid composition and maturation defects[S]

Content Provider	PubMed Central
Author	Blouin, Cédric M. Lay, Soazig Le Eberl, Anita Köfeler, Harald C. Guerrera, Ida Chiara Klein, Christophe Le, Liepvre Xavier Lasnier, Françoise Olivier, Bourron Gautier, Jean-françois Ferré, Pascal Hajduch, Eric Isabelle, Dugail
Copyright Year	2010
Abstract	Caveolins form plasmalemnal invaginated caveolae. They also locate around intracellular lipid droplets but their role in this location remains unclear. By studying primary adipocytes that highly express caveolin-1, we characterized the impact of caveolin-1 deficiency on lipid droplet proteome and lipidome. We identified several missing proteins on the lipid droplet surface of caveolin-deficient adipocytes and showed that the caveolin-1 lipid droplet pool is organized as multi-protein complexes containing cavin-1, with similar dynamics as those found in caveolae. On the lipid side, caveolin deficiency did not qualitatively alter neutral lipids in lipid droplet, but significantly reduced the relative abundance of surface phospholipid species: phosphatidylserine and lysophospholipids. Caveolin-deficient adipocytes can form only small lipid droplets, suggesting that the caveolin-lipid droplet pool might be involved in lipid droplet size regulation. Accordingly, we show that caveolin-1 concentration on adipocyte lipid droplets positively correlated with lipid droplet size in obese rodent models and human adipocytes. Moreover, rescue experiments by caveolin- green fluorescent protein in caveolin-deficient cells exposed to fatty acid overload demonstrated that caveolin-coated lipid droplets were able to grow larger than caveolin-devoid lipid droplets. Altogether, these data demonstrate that the lipid droplet-caveolin pool impacts on phospholipid and protein surface composition of lipid droplets and suggest a functional role on lipid droplet expandability.
Related Links	http://dx.doi.org/10.1194/jlr.m001016
Ending Page	956
Page Count	12
Starting Page	945
File Format	PDF
ISSN	00222275
e-ISSN	15397262
Journal	Journal of Lipid Research
Issue Number	5
Volume Number	51
Language	English
Publisher	The American Society for Biochemistry and Molecular Biology
Publisher Date	2010-05-01
Access Restriction	Open
Rights Holder	The American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Endocrinology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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