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| Content Provider | PubMed Central |
|---|---|
| Author | Yin, Xiaoke Cuello, Friederike Mayr, Ursula Hao, Zhiqi Hornshaw, Martin Ehler, Elisabeth Metin, Avkiran Mayr, Manuel |
| Abstract | Myofilament proteins are responsible for cardiac contraction. The myofilament subproteome, however, has not been comprehensively analyzed thus far. In the present study, cardiomyocytes were isolated from rodent hearts and stimulated with endothelin-1 and isoproterenol, potent inducers of myofilament protein phosphorylation. Subsequently, cardiomyocytes were “skinned,” and the myofilament subproteome was analyzed using a high mass accuracy ion trap tandem mass spectrometer (LTQ Orbitrap XL) equipped with electron transfer dissociation. As expected, a small number of myofilament proteins constituted the majority of the total protein mass with several known phosphorylation sites confirmed by electron transfer dissociation. More than 600 additional proteins were identified in the cardiac myofilament subproteome, including kinases and phosphatase subunits. The proteomic comparison of myofilaments from control and treated cardiomyocytes suggested that isoproterenol treatment altered the subcellular localization of protein phosphatase 2A regulatory subunit B56α. Immunoblot analysis of myocyte fractions confirmed that β-adrenergic stimulation by isoproterenol decreased the B56α content of the myofilament fraction in the absence of significant changes for the myosin phosphatase target subunit isoforms 1 and 2 (MYPT1 and MYPT2). Furthermore, immunolabeling and confocal microscopy revealed the spatial redistribution of these proteins with a loss of B56α from Z-disc and M-band regions but increased association of MYPT1/2 with A-band regions of the sarcomere following β-adrenergic stimulation. In summary, we present the first comprehensive proteomics data set of skinned cardiomyocytes and demonstrate the potential of proteomics to unravel dynamic changes in protein composition that may contribute to the neurohormonal regulation of myofilament contraction. |
| Related Links | http://dx.doi.org/10.1074/mcp.m900275-mcp200 |
| Ending Page | 509 |
| Page Count | 13 |
| Starting Page | 497 |
| File Format | |
| ISSN | 15359476 |
| e-ISSN | 15359484 |
| Journal | Molecular & Cellular Proteomics : MCP |
| Issue Number | 3 |
| Volume Number | 9 |
| Language | English |
| Publisher | The American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2010-03-01 |
| Access Restriction | Open |
| Rights Holder | The American Society for Biochemistry and Molecular Biology |
| Subject Keyword | Analytical Chemistry Biochemistry Molecular Biology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Molecular Biology Biochemistry |
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