NDLI: Progesterone and 17β-Estradiol Enhance Regulatory Responses to Human Papillomavirus Type 16 Virus-Like Particles in Peripheral Blood Mononuclear Cells from Healthy Women▿

Content Provider	PubMed Central
Author	Marks, Morgan A. Gravitt, Patti E. Burk, Robert D. Studentsov, Yevgeniy Homayoon, Farzadegan Klein, Sabra L.
Copyright Year	2010
Abstract	Human papillomavirus (HPV) virus-like particle (VLP) vaccines are highly effective at preventing viral infections and the development of precancerous lesions through the induction of high-titer neutralizing antibodies and strong cell-mediated immune responses. Women taking combined oral contraceptives (COCs), however, show large variabilities in the magnitudes of their antibody responses. The goal of the present study was to determine the effects of 17β-estradiol (E2) and progesterone (P4) alone and in combination on the cellular immune response to HPV type 16 (HPV-16) VLPs in vitro. Peripheral blood mononuclear cells (PBMCs) from healthy donor women were stimulated in vitro with HPV-16 VLPs (2.5 μg/ml) in the presence of E2 and P4 administered either alone or in combination; and lymphoproliferation, cytokine production, transcription factor expression, and steroid hormone receptor expression were analyzed. HPV-16 VLPs significantly increased the levels of lymphoproliferation, proinflammatory cytokine (gamma interferon [IFN-γ], interleukin-1β [IL-1β], IL-2, IL-6, IL-8, IL-12p70, IL-17, tumor necrosis factor alpha [TNF-α]) production, anti-inflammatory cytokine (IL-1ra, IL-10) production, and the expression of Erα and Erβ but decreased the levels of Foxp3 expression and production of transforming growth factor β (TGF-β). Exposure of PBMCs to E2 and P4 either alone or in combination significantly decreased the levels of lymphoproliferation and production of proinflammatory cytokines (IFN-γ, IL-12p70, TNF-α) but increased the levels of production of IL-10 and TGF-β and the expression of Foxp3 in response to HPV-16 VLPs. Treatment of cells with biologically relevant concentrations of sex steroid hormones suppressed the inflammatory response and enhanced the regulatory response to HPV-16 VLPs, which may have implications for predicting the long-term efficacy of HPV vaccines, adverse events, and cross-protection among women taking COCs.
Related Links	http://dx.doi.org/10.1128/cvi.00441-09
Ending Page	617
Page Count	9
Starting Page	609
File Format	PDF
ISSN	15566811
e-ISSN	1556679X
Journal	Clinical and Vaccine Immunology : CVI
Issue Number	4
Volume Number	17
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2010-04-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Immunology Immunology and Allergy Microbiology (medical) Clinical Biochemistry Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Clinical Biochemistry Immunology Microbiology (medical)

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