NDLI: Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes

Content Provider	PubMed Central
Author	Grünberg, Sebastian Reich, Christoph Zeller, Mirijam E. Bartlett, Michael S. Michael, Thomm
Copyright Year	2009
Abstract	The lower jaws of archaeal RNA polymerase and eukaryotic RNA polymerase II include orthologous subunits H and Rpb5, respectively. The tertiary structure of H is very similar to the structure of the C-terminal domain of Rpb5, and both subunits are proximal to downstream DNA in pre-initiation complexes. Analyses of reconstituted euryarchaeal polymerase lacking subunit H revealed that H is important for open complex formation and initial transcription. Eukaryotic Rpb5 rescues activity of the ΔH enzyme indicating a strong conservation of function for this subunit from archaea to eukaryotes. Photochemical cross-linking in elongation complexes revealed a striking structural rearrangement of RNA polymerase, bringing subunit H near the transcribed DNA strand one helical turn downstream of the active center, in contrast to the positioning observed in preinitiation complexes. The rearrangement of subunits H and A′′ suggest a major conformational change in the archaeal RNAP lower jaw upon formation of the elongation complex.
Related Links	http://dx.doi.org/10.1093/nar/gkp1190
Starting Page	1950
File Format	PDF
ISSN	13624962
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	6
Volume Number	38
Language	English
Publisher	Oxford University Press
Publisher Date	2010-04-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes

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Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes

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Rearrangement of the RNA polymerase subunit H and the lower jaw in archaeal elongation complexes

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