NDLI: Molecular keys of the tropism of integration of the cholera toxin phage

Content Provider	PubMed Central
Author	Das, Bhabatosh Julien, Bischerour Val, Marie-eve Barre, François-xavier
Abstract	Cholera toxin is encoded in the genome of CTXφ, a lysogenic filamentous phage of Vibrio cholerae. CTXφ variants contribute to the genetic diversity of cholera epidemic strains. It has been shown that the El Tor variant of CTXφ hijacks XerC and XerD, two host-encoded tyrosine recombinases that normally function to resolve chromosome dimers, to integrate at dif1, the dimer resolution site of the larger of the two V. cholerae chromosomes. However, the exact mechanism of integration of CTXφ and the rules governing its integration remained puzzling, with phage variants integrated at either or both dimer resolution sites of the two V. cholerae chromosomes. We designed a genetic system to determine experimentally the tropism of integration of CTXφ and thus define rules of compatibility between phage variants and dimer resolution sites. We then showed in vitro how these rules are explained by the direct integration of the single-stranded phage genome into the double-stranded bacterial genome. Finally, we showed how the evolution of phage attachment and chromosome dimer resolution sites contributes to the generation of genetic diversity among cholera epidemic strains.
Related Links	http://dx.doi.org/10.1073/pnas.0910212107
Ending Page	4382
Page Count	6
Starting Page	4377
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	107
Language	English
Publisher	National Academy of Sciences
Publisher Date	2010-03-02
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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