NDLI: Complex relationship between TCTP, microtubules and actin microfilaments regulates cell shape in normal and cancer cells

Content Provider	PubMed Central
Author	Bazile, Franck Pascal, Aude Arnal, Isabelle Le, Clainche Christophe Franck, Chesnel Kubiak, Jacek Z.
Abstract	Translationally Controlled Tumor-associated Protein (TCTP) is a ubiquitous and highly conserved protein implicated in cancers. Here we demonstrate that interactions of TCTP with microtubules (MTs) are functionally important but indirect, and we reveal novel interaction of TCTP with the actin cytoskeleton. Firstly, immunofluorescence in Xenopus XL2 cells revealed cytoplasmic fibers stained with TCTP but not with tubulin antibodies, as well as MT-free of TCTP. Furthermore, TCTP localized to a subset of actin-rich fibers in migrating cells. Secondly XlTCTP did not affect in vitro assembly/disassembly of MTs, and lacked MT binding affinity both in pull-down assays and in cell-free extracts. Although TCTP also failed to bind to purified F-actin, it associated with microfilaments in cell-free extracts. Thirdly, TCTP concentrated in mitotic spindle did not colocalize with MTs, and was easily dissociated from these structures except at the poles. Finally, RNAi knockdown of TCTP in XL2 and HeLa cells provoked drastic, MT-dependent, shape change. These data show that although TCTP interacts with MTs it does not behave as classic MT Associated Protein (MAP). Our evidence for an association of TCTP with F-actin structures, and for an involvement in cell shape regulation, implicates this protein in integrating cytoskeletal interations both in interphase and mitosis providing a new avenue to fully understand the role of TCTP.
Related Links	http://dx.doi.org/10.1093/carcin/bgp022
Ending Page	565
Page Count	11
Starting Page	555
File Format	PDF
ISSN	01433334
e-ISSN	14602180
Journal	Carcinogenesis
Issue Number	4
Volume Number	30
Language	English
Publisher	Oxford University Press
Publisher Date	2009-01-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Cancer Research Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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