NDLI: ARS5 is a component of the 26S proteasome complex and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis

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ARS5 is a component of the 26S proteasome complex and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis

Content Provider	PubMed Central
Author	Sung, Dong-yul Kim, Tae-houn Komives, Elizabeth A. Mendoza-cózatl, David G. Schroeder, Julian I.
Abstract	A forward genetic screen in Arabidopsis led to the isolation of several arsenic tolerance mutants. ars5 is the strongest arsenate and arsenite resistant mutant identified in this genetic screen. Here, we report the characterization and cloning of the ars5 mutant gene. ars5 is shown to exhibit an increased accumulation of arsenic and thiol compounds during arsenic stress. Rough mapping together with microarray-based expression mapping identified the ars5 mutation in the alpha subunit F (PAF1) of the 26S proteasome complex. Characterization of an independent paf1 T-DNA insertion allele and complementation by PAF1 confirmed that paf1 mutation is responsible for the enhanced thiol accumulation and the arsenic tolerance phenotypes. Arsenic tolerance was not observed in a knockout mutant of the highly homologous PAF2 gene. However, genetic complementation of ars5 by over expression of PAF2 suggests that the PAF2 protein is functionally equivalent to PAF1 when expressed at high levels. No detectible difference was observed in total ubiquitinylated protein profiles between ars5 and wild type Arabidopsis, suggesting that the arsenic tolerance observed in ars5 is not derived from a general impairment in proteasome-mediated protein degradation. Quantitative RT-PCR showed that arsenic induces enhanced transcriptional activation of several key genes that function in glutathione and phytochelatin biosynthesis in wild type and this arsenic-induction of gene expression is more dramatic in ars5. The enhanced transcriptional response to arsenic and the increased accumulation of thiol compounds in ars5 compared to WT suggest the presence of a positive regulation pathway for thiol biosynthesis that is enhanced in the ars5 background.
Related Links	http://dx.doi.org/10.1111/j.1365-313x.2009.03914.x
Ending Page	813
Page Count	12
Starting Page	802
File Format	PDF
ISSN	09607412
e-ISSN	1365313X
Journal	The Plant journal : for cell and molecular biology
Issue Number	5
Volume Number	59
Language	English
Publisher Date	2009-09-01
Access Restriction	Open
Subject Keyword	Plant Science Genetics Cell Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Plant Science

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