NDLI: Real-Time Imaging of Cellulose Reorientation during Cell Wall Expansion in Arabidopsis Roots1 [W] [OA]

Content Provider	PubMed Central
Author	Anderson, Charles T. Carroll, Andrew Laila, Akhmetova Somerville, Chris
Copyright Year	2010
Abstract	Cellulose forms the major load-bearing network of the plant cell wall, which simultaneously protects the cell and directs its growth. Although the process of cellulose synthesis has been observed, little is known about the behavior of cellulose in the wall after synthesis. Using Pontamine Fast Scarlet 4B, a dye that fluoresces preferentially in the presence of cellulose and has excitation and emission wavelengths suitable for confocal microscopy, we imaged the architecture and dynamics of cellulose in the cell walls of expanding root cells. We found that cellulose exists in Arabidopsis (Arabidopsis thaliana) cell walls in large fibrillar bundles that vary in orientation. During anisotropic wall expansion in wild-type plants, we observed that these cellulose bundles rotate in a transverse to longitudinal direction. We also found that cellulose organization is significantly altered in mutants lacking either a cellulose synthase subunit or two xyloglucan xylosyltransferase isoforms. Our results support a model in which cellulose is deposited transversely to accommodate longitudinal cell expansion and reoriented during expansion to generate a cell wall that is fortified against strain from any direction.
Related Links	http://dx.doi.org/10.1104/pp.109.150128
Ending Page	796
Page Count	10
Starting Page	787
File Format	PDF
ISSN	00320889
e-ISSN	15322548
Journal	Plant Physiology
Issue Number	2
Volume Number	152
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2010-02-01
Access Restriction	Open
Rights Holder	American Society of Plant Biologists
Subject Keyword	Plant Science Genetics Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Real-Time Imaging of Cellulose Reorientation during Cell Wall Expansion in Arabidopsis Roots

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Real-Time Imaging of Cellulose Reorientation during Cell Wall Expansion in Arabidopsis Roots

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Nitrite Acts as a Transcriptome Signal at Micromolar Concentrations in Arabidopsis Roots1 [W] [OA]

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CELLULOSE SYNTHASE9 Serves a Nonredundant Role in Secondary Cell Wall Synthesis in Arabidopsis Epidermal Testa Cells 1 [C] [W] [OA]

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