NDLI: Effect of a tapasin mutant on the assembly of the mouse MHC class I molecule H2-Kd

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Effect of a tapasin mutant on the assembly of the mouse MHC class I molecule H2-Kd

Content Provider	PubMed Central
Author	Simone, Laura C. Wang, Xiaojian Tuli, Amit Solheim, Joyce C.
Abstract	Major histocompatibility complex (MHC) class I heavy chain/β2m heterodimers assemble with antigenic peptides via interactions with peptide-loading complex proteins, including tapasin and ERp57. In human cells, a cysteine residue within tapasin (C95) has been shown to form a covalent bond with ERp57. In this study, we focused on the effect of this tapasin amino acid residue in mouse cells expressing the MHC class I molecule H2-Kd. We demonstrated that a large disulfide-bonded complex was present in the mouse cells that included ERp57, tapasin, and Kd. Furthermore, in mouse cells, unlike human cells, we found that tapasin mutated at C95 can participate in a non-covalent complex with ERp57. Comparison of our findings to earlier findings with a human molecule (HLA-B4402) also revealed that a tapasin C95 mutation has a stronger effect on the maturation and stability of Kd than HLA-B4402. Overall, our results characterize the influence of this tapasin cysteine residue on the stable surface expression of a mouse MHC class I molecule, and reveal differences in tapasin C95 interactions and effects between mouse and human systems.
Related Links	http://dx.doi.org/10.1038/icb.2009.59
Ending Page	62
Page Count	6
Starting Page	57
File Format	PDF
ISSN	08189641
e-ISSN	14401711
Journal	Immunology and cell biology
Issue Number	1
Volume Number	88
Language	English
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Immunology Cell Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Immunology and Allergy Immunology

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Sequence, linkage to H2-K, and function of mouse tapasin in MHC class I assembly

A transmembrane tail: interaction of tapasin with TAP and the MHC class I molecule

Stoichiometric tapasin interactions in the catalysis of major histocompatibility complex class I molecule assembly

Influence of the tapasin C terminus on the assembly of MHC class I allotypes

Influence of the tapasin C-terminus on the assembly of MHC class I allotypes

Amyloid Precursor-like Protein 2 Increases the Endocytosis, Instability, and Turnover of the H2-Kd MHC Class I Molecule1

MECHANISMS OF FUNCTION OF TAPASIN, A CRITICAL MHC CLASS I ASSEMBLY FACTOR

Assembly of tapasin-associated MHC class I in the absence of the transporter associated with antigen processing (TAP)

Distinct functions for the glycans of tapasin and heavy chains in the assembly of major histocompatibility complex (MHC) class I molecules

Effect of a tapasin mutant on the assembly of the mouse MHC class I molecule H2-Kd

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