NDLI: Telomeric circles are abundant in the stn1-M1 mutant that maintains its telomeres through recombination

Content Provider	PubMed Central
Author	Basenko, Evelina Y. Cesare, Anthony J. Iyer, Shilpa Griffith, Jack D. Mceachern, Michael J.
Copyright Year	2009
Abstract	Some human cancers maintain their telomeres using the alternative lengthening of telomeres (ALT) mechanism; a process thought to involve recombination. Different types of recombinational telomere elongation pathways have been identified in yeasts. In senescing yeast telomerase deletion (ter1-Δ) mutants with very short telomeres, it has been hypothesized that copying a tiny telomeric circle (t-circle) by a rolling circle mechanism is the key event in telomere elongation. In other cases more closely resembling ALT cells, such as the stn1-M1 mutant of Kluyveromyces lactis, the telomeres appear to be continuously unstable and routinely reach very large sizes. By employing two-dimensional gel electrophoresis and electron microscopy, we show that stn1-M1 cells contain abundant double stranded t-circles ranging from ∼100 to 30 000 bp in size. We also observed small single-stranded t-circles, specifically composed of the G-rich telomeric strand and tailed circles resembling rolling circle replication intermediates. The t-circles most likely arose from recombination events that also resulted in telomere truncations. The findings strengthen the possibility that t-circles contribute to telomere maintenance in stn1-M1 and ALT cells.
Related Links	http://dx.doi.org/10.1093/nar/gkp814
Ending Page	189
Page Count	8
Starting Page	182
File Format	PDF
ISSN	03051048
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	1
Volume Number	38
Language	English
Publisher	Oxford University Press
Publisher Date	2010-01-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

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Telomeric circles are abundant in the stn1-M1 mutant that maintains its telomeres through recombination

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