NDLI: Host Chemokine and Cytokine Response in the Endocervix within the First Developmental Cycle of Chlamydia muridarum ▿

Content Provider	PubMed Central
Author	Rank, Roger G. Lacy, H. Marie Goodwin, Anna Sikes, James Judy, Whittimore Wyrick, Priscilla B. Nagarajan, Uma M.
Copyright Year	2010
Abstract	The initial host response in a primary chlamydial infection is the onset of acute inflammation. However, we still know very little about the early temporal events in the induction of the acute inflammatory response and how these events relate to the initial chlamydial developmental cycle in an actual genital infection. Because it was critical to initiate a synchronous infection in the endocervix in the first 24 h to evaluate the sequential expression of the host response, we developed the surgical methodology of depositing Chlamydia muridarum directly on the endocervix. Cervical tissue was collected at 3, 12, and 24 h after inoculation and the expression array of chemokines, cytokines, and receptors was assessed to characterize the response during the initial developmental cycle. Polymorphonuclear leukocyte (PMN) infiltration was first observed at 12 h after inoculation, and a few PMNs could be seen in the epithelium at 24 h. Electron microscopic analysis at 24 h showed that virtually all inclusions were at the same stage of development, indicating a synchronous infection. Several chemokine and cytokine genes were expressed as early as 3 h after infection, but by 12 h, 41 genes were expressed. Thus, activation of the host response occurs both with the introduction of elementary bodies into the host and early replication of reticulate bodies. No significant response was observed when UV-inactivated organisms were inoculated into the cervix at any time interval. This model provides an ideal opportunity to investigate the mechanisms by which the early inflammatory response is induced in vivo.
Related Links	http://dx.doi.org/10.1128/iai.00772-09
Ending Page	544
Page Count	9
Starting Page	536
File Format	PDF
ISSN	00199567
e-ISSN	10985522
Journal	Infection and Immunity
Issue Number	1
Volume Number	78
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2010-01-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Immunology Microbiology Parasitology Infectious Diseases Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Parasitology Immunology Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Survival of Chlamydia muridarum within Dendritic Cells▿

Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix.

Murine Oviduct Epithelial Cell Cytokine Responses to Chlamydia muridarum Infection Include Interleukin-12-p70 Secretion

Mutational Analysis of the Chlamydia muridarum Plasticity Zone

Chlamydia muridarum Induction of Glandular Duct Dilation in Mice

Developmental cycle-specific host-free RNA synthesis in Chlamydia spp.

The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection

The Recall Response Induced by Genital Challenge with Chlamydia muridarum Protects the Oviduct from Pathology but Not from Reinfection

Chemokine Expression Patterns Differ within Anatomically Distinct Regions of the Genital Tract during Chlamydia trachomatis Infection

Host Chemokine and Cytokine Response in the Endocervix within the First Developmental Cycle of Chlamydia muridarum ▿

Similar Documents

Survival of Chlamydia muridarum within Dendritic Cells▿

Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix.

Murine Oviduct Epithelial Cell Cytokine Responses to Chlamydia muridarum Infection Include Interleukin-12-p70 Secretion

Mutational Analysis of the Chlamydia muridarum Plasticity Zone

Chlamydia muridarum Induction of Glandular Duct Dilation in Mice

Developmental cycle-specific host-free RNA synthesis in Chlamydia spp.

The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection

The Recall Response Induced by Genital Challenge with Chlamydia muridarum Protects the Oviduct from Pathology but Not from Reinfection

Chemokine Expression Patterns Differ within Anatomically Distinct Regions of the Genital Tract during Chlamydia trachomatis Infection

Host Chemokine and Cytokine Response in the Endocervix within the First Developmental Cycle of Chlamydia muridarum ▿