NDLI: New Methods for Chicken Embryo Manipulations

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New Methods for Chicken Embryo Manipulations

Content Provider	PubMed Central
Author	El Ghali, Nissrine Maes, Rabadi Max, Ezin Bellard, Maria Elena De
Abstract	The capacity to image a growing embryo while simultaneously studying the developmental function of specific molecules provides invaluable information on embryogenesis. However, until recently, this approach was accomplished with difficulty both because of the advanced technology needed and because an easy method of minimizing damage to the embryo was unavailable. Here we present a novel way of adapting the well-known EC culture of whole chick embryos to time-lapse imaging and to functional molecular studies using blocking agents. The novelty of our method stems from the ability to apply blocking agents ex ovo as well as in ovo. We were able to study the function of a set of molecules by culturing developing embryos ex ovo in tissue culture media containing these molecules or by injecting them underneath the live embryo in ovo. The in ovo preparation is particularly valuable since it extends the period of time during which the developmental function of the molecule can be studied and it provides an easy, reproducible method for screening a batch of molecules. These new techniques will prove very helpful in visualizing and understanding the role of specific molecules during embryonic morphogenesis, including blood vessel formation.
Related Links	http://dx.doi.org/10.1002/jemt.20753
Page Count	1
Starting Page	58
File Format	PDF
ISSN	1059910X
e-ISSN	10970029
Journal	Microscopy research and technique
Issue Number	1
Volume Number	73
Language	English
Publisher Date	2009-01-01
Access Restriction	Open
Subject Keyword	Anatomy Instrumentation Medical Laboratory Technology Histology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Anatomy Histology Instrumentation Medical Laboratory Technology

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