NDLI: Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA

Content Provider	PubMed Central
Author	Djupedal, Ingela Kos-braun, Isabelle C. Mosher, Rebecca A. Söderholm, Niklas Simmer, Femke Hardcastle, Thomas J. Fender, Aurélie Heidrich, Nadja Alexander, Kagansky Bayne, Elizabeth Wagner, E. Gerhart H. Baulcombe, David C. Allshire, Robin C. Ekwall, Karl
Copyright Year	2009
Abstract	The formation of heterochromatin at the centromeres in fission yeast depends on transcription of the outer repeats. These transcripts are processed into siRNAs that target homologous loci for heterochromatin formation. Here, high throughput sequencing of small RNA provides a comprehensive analysis of centromere-derived small RNAs. We found that the centromeric small RNAs are Dcr1 dependent, carry 5′-monophosphates and are associated with Ago1. The majority of centromeric small RNAs originate from two remarkably well-conserved sequences that are present in all centromeres. The high degree of similarity suggests that this non-coding sequence in itself may be of importance. Consistent with this, secondary structure-probing experiments indicate that this centromeric RNA is partially double-stranded and is processed by Dicer in vitro. We further demonstrate the existence of small centromeric RNA in rdp1Δ cells. Our data suggest a pathway for siRNA generation that is distinct from the well-documented model involving RITS/RDRC. We propose that primary transcripts fold into hairpin-like structures that may be processed by Dcr1 into siRNAs, and that these siRNAs may initiate heterochromatin formation independent of RDRC activity.
Related Links	http://dx.doi.org/10.1038/emboj.2009.351
Ending Page	3844
Page Count	13
Starting Page	3832
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	24
Volume Number	28
Language	English
Publisher	Nature Publishing Group
Publisher Date	2009-12-16
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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