NDLI: Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs

Content Provider	PubMed Central
Author	Onyshchenko, Mykola I. Gaynutdinov, Timur I. Englund, Ethan A. Appella, Daniel H. Neumann, Ronald D. Panyutin, Igor G.
Copyright Year	2009
Abstract	Numerous regulatory genes have G-rich regions that can potentially form quadruplex structures, possibly playing a role in transcription regulation. We studied a G-rich sequence in the BCL2 gene 176-bp upstream of the P1 promoter for G-quadruplex formation. Using circular dichroism (CD), thermal denaturation and dimethyl sulfate (DMS) footprinting, we found that a single-stranded oligonucleotide with the sequence of the BCL2 G-rich region forms a potassium-stabilized G-quadruplex. To study G-quadruplex formation in double-stranded DNA, the G-rich sequence of the BCL2 gene was inserted into plasmid DNA. We found that a G-quadruplex did not form in the insert at physiological conditions. To induce G-quadruplex formation, we used short peptide nucleic acids (PNAs) that bind to the complementary C-rich strand. We examined both short duplex-forming PNAs, complementary to the central part of the BCL2 gene, and triplex-forming bis-PNAs, complementary to sequences adjacent to the G-rich BCL2 region. Using a DMS protection assay, we demonstrated G-quadruplex formation within the G-rich sequence from the promoter region of the human BCL2 gene in plasmid DNA. Our results show that molecules binding the complementary C-strand facilitate G-quadruplex formation and introduce a new mode of PNA-mediated sequence-specific targeting.
Related Links	http://dx.doi.org/10.1093/nar/gkp840
Ending Page	7580
Page Count	11
Starting Page	7570
File Format	PDF
ISSN	03051048
e-ISSN	13624962
Journal	Nucleic Acids Research
Issue Number	22
Volume Number	37
Language	English
Publisher	Oxford University Press
Publisher Date	2009-12-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Genetics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs

NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region

Quadruplex formation is necessary for stable PNA invasion into duplex DNA of BCL2 promoter region

NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region

Regulation of PDGFR-β gene expression by targeting the G-vacancy bearing G-quadruplex in promoter

Quantitative analysis and prediction of G-quadruplex forming sequences in double-stranded DNA

Existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy

Existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy

Interaction of TMPyP4, TMPyP3, and TMPyP2 with Intramolecular G-Quadruplex Formed by Promoter Region of Bcl2 and KRAS NHPPE

Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs

Similar Documents

Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs

NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region

Quadruplex formation is necessary for stable PNA invasion into duplex DNA of BCL2 promoter region

NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region

Regulation of PDGFR-β gene expression by targeting the G-vacancy bearing G-quadruplex in promoter

Quantitative analysis and prediction of G-quadruplex forming sequences in double-stranded DNA

Existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy

Existence of G-quadruplex structures in promoter region of oncogenes confirmed by G-quadruplex DNA cross-linking strategy

Interaction of TMPyP4, TMPyP3, and TMPyP2 with Intramolecular G-Quadruplex Formed by Promoter Region of Bcl2 and KRAS NHPPE

Stabilization of G-quadruplex in the BCL2 promoter region in double-stranded DNA by invading short PNAs