NDLI: Characterization of Protein Aggregation via Intrinsic Fluorescence Lifetime

Content Provider	PubMed Central
Author	Schlick, Kristian H. Lange, Candace K. Gillispie, Gregory D. Cloninger, Mary J.
Abstract	Aggregation plays an integral role in multivalent protein-carbohydrate interactions, in Alzheimer’s and other amyloid-related diseases, and in infection response. Efforts have been made to apply controlled-aggregation in toxin sensors. We have developed a label-free intrinsic fluorescence lifetime assay that uniquely can monitor aggregation processes in real time without interference from precipitation. Fluorescence decay curves were measured with high precision at one-second time intervals following addition of a glycodendrimer to a lectin-containing solution. Changes in the fluorescence intensity and lifetime signified formation of complexes. However, these changes are not associated with the initial lectin-sugar binding events. Rather, they appear to be caused by clustering and a subsequent conformational rearrangement of the lectins. Studies were conducted with mannose-functionalized PAMAM dendrimers of the second through the sixth generations with Concanavalin A. The apparent rate constant, when expressed on a per mannose basis, increased with dendrimer generation, particularly in going from the fourth to the sixth generation. However, the identical fluorescence decay waveforms for saturating amounts of dendrimer suggest that all glycodendrimer generations studied reach a comparable state of aggregation. Although self-quenching of tryptophan resonances that was induced by clustering was monitored in this study, the reported method is not limited to such and is viable for numerous binding studies.
Related Links	http://dx.doi.org/10.1021/ja904073p
Ending Page	16609
Page Count	2
Starting Page	16608
File Format	PDF
ISSN	00027863
e-ISSN	15205126
Journal	Journal of the American Chemical Society
Issue Number	46
Volume Number	131
Language	English
Publisher Date	2009-11-25
Access Restriction	Open
Subject Keyword	Colloid and Surface Chemistry Biochemistry Chemistry(all) Catalysis Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Colloid and Surface Chemistry Biochemistry Catalysis

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