NDLI: Identification of New Proteins in Follicular Fluid from Mature Human Follicles by Direct Sample Rehydration Method of Two-Dimensional Polyacrylamide Gel Electrophoresis

Content Provider	PubMed Central
Author	Lee, Han-chul Lee, Sang-wha Lee, Kyo Won Lee, Sook-whan Cha, Kwang-yul Kim, Kye Hyun Lee, Suman
Copyright Year	2005
Abstract	Human follicular fluid (HFF) includes various biologically active proteins which can affect follicle growth and oocyte fertilization. Thus far, these proteins from mature follicles in human follicular fluid have been poorly characterized. Here, two-dimensional polyacrylamide gel electrophoresis (2-DE) with matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) was used to identify new proteins in HFF. Mature follicular fluids were obtained from five females after oocyte collection during in vitro fertilization (IVF). We directly rehydrated HFF samples, obtained high-resolution 2-DE maps, and processed them for 2-DE and MALDI-MS. One hundred eighty spots were detected and 10 of these spots were identified. By the 2-DE database, six of them had been reported, as proteins already existing in HFF. Hormone sensitive lipase (HSL), Unnamed protein product 1 (UPP1), Unnamed protein product 2 (UPP2), and apolipoprotein A-IV precursor were newly detected. HSL and apolipoprotein A-IV participate in lipid metabolism. UPP1 has a homology with selenocysteine lyase. We found by RT-PCR that these genes are expressed from human primary granulosa cells. The proteins identified here may emerge as potential candidates for specific functions during folliculogenesis, hormone secretion regulation, or oocyte maturation. Further functional analysis of these proteins is necessitated to determine their biological implications.
Related Links	http://dx.doi.org/10.3346/jkms.2005.20.3.456
Starting Page	456
File Format	PDF
ISSN	10118934
e-ISSN	15986357
Journal	Journal of Korean Medical Science
Issue Number	3
Volume Number	20
Language	English
Publisher	The Korean Academy of Medical Sciences
Publisher Date	2005-01-01
Access Restriction	Open
Rights Holder	The Korean Academy of Medical Sciences
Subject Keyword	Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine

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