NDLI: Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption

Please wait, while we are loading the content...

Proinsulin C-peptide reduces diabetes-induced glomerular hyperfiltration via efferent arteriole dilation and inhibition of tubular sodium reabsorption

Early monitoring of acute tubular necrosis in the rat kidney by 23Na-MRI

Analysis of the cytoplasmic interaction between polycystin-1 and polycystin-2

Rac1 promotes TGF-β-stimulated mesangial cell type I collagen expression through a PI3K/Akt-dependent mechanism

Decreased renal perfusion rapidly increases plasma membrane Na-K-ATPase in rat cortex by an angiotensin II-dependent mechanism

The instructive role of metanephric mesenchyme in ureteric bud patterning, sculpting, and maturation and its potential ability to buffer ureteric bud branching defects

AT1 receptor-mediated uptake of angiotensin II and NHE-3 expression in proximal tubule cells through a microtubule-dependent endocytic pathway

Transglutaminase-1 protects renal epithelial cells from hydrogen peroxide-induced apoptosis through activation of STAT3 and AKT signaling pathways

Oxalate-induced activation of PKC-α and -δ regulates NADPH oxidase-mediated oxidative injury in renal tubular epithelial cells

Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption

Reduction of renal triglyceride accumulation: effects on proximal tubule Na+/H+ exchange and urinary acidification

Developmental delays consistent with cochlear hypothyroidism contribute to failure to develop hearing in mice lacking Slc26a4/pendrin expression

Early exposure to germs modifies kidney damage and inflammation after experimental ischemia-reperfusion injury

Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate

Year: 2009, Volume: 297, Issue: 4

Year: 2009, Volume: 297, Issue: 3

Year: 2009, Volume: 297, Issue: 2

Year: 2009, Volume: 297, Issue: 1

Year: 2009, Volume: 296

Year: 2008, Volume: 296

Year: 2008, Volume: 295

Year: 2008, Volume: 294

Year: 2007, Volume: 293

Year: 2003, Volume: 285

Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption

Content Provider	PubMed Central
Author	Bugaj, Vladislav Pochynyuk, Oleh Stockand, James D.
Abstract	We used patch-clamp electrophysiology on isolated, split-open murine collecting ducts (CD) to test the hypothesis that regulation of epithelial sodium channel (ENaC) activity is a physiologically important effect of vasopressin. Surprisingly, this has not been tested directly before. We ask whether vasopressin affects ENaC activity distinguishing between acute and chronic effects, as well as, parsing the cellular signaling pathway and molecular mechanism of regulation. In addition, we quantified possible synergistic regulation of ENaC by vasopressin and aldosterone associating this with a requirement for distal nephron Na+ reabsorption during water conservation vs. maintenance of Na+ balance. We find that vasopressin significantly increases ENaC activity within 2–3 min by increasing open probability (P o). This activation was dependent on adenylyl cyclase (AC) and PKA. Water restriction (18–24 h) and pretreatment of isolated CD with vasopressin (∼30 min) resulted in a similar increase in P o. In addition, this also increased the number (N) of active ENaC in the apical membrane. Similar to P o, increases in N were sensitive to inhibitors of AC. Stressing animals with water and salt restriction separately and jointly revealed an important effect of vasopressin: conservation of water and Na+ each independently increased ENaC activity and jointly had a synergistic effect on channel activity. These results demonstrate a quantitatively important action of vasopressin on ENaC suggesting that distal nephron Na+ reabsorption mediated by this channel contributes to maintenance of water reabsorption. In addition, our results support that the combined actions of vasopressin and aldosterone are required to achieve maximally activated ENaC.
Related Links	http://dx.doi.org/10.1152/ajprenal.00371.2009
Starting Page	1411
File Format	PDF
ISSN	03636127
e-ISSN	15221466
Journal	American Journal of Physiology - Renal Physiology
Issue Number	5
Volume Number	297
Language	English
Publisher	American Physiological Society
Publisher Date	2009-11-01
Access Restriction	Open
Rights Holder	American Physiological Society
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Activation of the epithelial $Na^{+}$ channel in the collecting duct by vasopressin contributes to water reabsorption

Protease stimulation of renal sodium reabsorption in vivo by activation of the collecting duct epithelial sodium channel (ENaC)

Regulation of the epithelial Na+ channel by endothelin-1 in rat collecting duct

Oxygen regulation of the epithelial Na channel in the collecting duct

Regulation of collecting duct water channel expression by vasopressin in Brattleboro rat.

Apelin Counteracts Vasopressin-Induced Water Reabsorption via Cross Talk Between Apelin and Vasopressin Receptor Signaling Pathways in the Rat Collecting Duct

A hypothesis linking sodium and lithium reabsorption in the distal nephron

Role of BKβ1 in Na+ reabsorption by cortical collecting ducts of Na+-deprived mice

Fluvastatin modulates renal water reabsorption in vivo through increased AQP2 availability at the apical plasma membrane of collecting duct cells

Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption

Similar Documents

Activation of the epithelial $Na^{+}$ channel in the collecting duct by vasopressin contributes to water reabsorption

Protease stimulation of renal sodium reabsorption in vivo by activation of the collecting duct epithelial sodium channel (ENaC)

Regulation of the epithelial Na+ channel by endothelin-1 in rat collecting duct

Oxygen regulation of the epithelial Na channel in the collecting duct

Regulation of collecting duct water channel expression by vasopressin in Brattleboro rat.

Apelin Counteracts Vasopressin-Induced Water Reabsorption via Cross Talk Between Apelin and Vasopressin Receptor Signaling Pathways in the Rat Collecting Duct

A hypothesis linking sodium and lithium reabsorption in the distal nephron

Role of BKβ1 in Na+ reabsorption by cortical collecting ducts of Na+-deprived mice

Fluvastatin modulates renal water reabsorption in vivo through increased AQP2 availability at the apical plasma membrane of collecting duct cells

Activation of the epithelial Na+ channel in the collecting duct by vasopressin contributes to water reabsorption