NDLI: Electrostatic interactions between rotor and stator in the bacterial flagellar motor

Content Provider	PubMed Central
Author	Zhou, Jiadong Lloyd, Scott A. Blair, David F.
Copyright Year	1998
Abstract	Bacterial flagellar motors rotate, obtaining power from the membrane gradient of protons or, in some species, sodium ions. Torque generation in the flagellar motor must involve interactions between components of the rotor and components of the stator. Sites of interaction between the rotor and stator have not been identified. Mutational studies of the rotor protein FliG and the stator protein MotA showed that both proteins contain charged residues essential for motor rotation. This suggests that functionally important electrostatic interactions might occur between the rotor and stator. To test this proposal, we examined double mutants with charged-residue substitutions in both the rotor protein FliG and the stator protein MotA. Several combinations of FliG mutations with MotA mutations exhibited strong synergism, whereas others showed strong suppression, in a pattern that indicates that the functionally important charged residues of FliG interact with those of MotA. These results identify a functionally important site of interaction between the rotor and stator and suggest a hypothesis for electrostatic interactions at the rotor–stator interface.
Starting Page	6436
File Format	PDF
ISSN	10916490
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	11
Volume Number	95
Language	English
Publisher	The National Academy of Sciences
Publisher Date	1998-05-26
Access Restriction	Open
Rights Holder	The National Academy of Sciences
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Structure and Function of the Bi-Directional Bacterial Flagellar Motor

Site-directed crosslinking identifies the stator-rotor interaction surfaces in a hybrid bacterial flagellar motor

Motility protein interactions in the bacterial flagellar motor.

Gate-controlled proton diffusion and protonation-induced ratchet motion in the stator of the bacterial flagellar motor

Torque and switching in the bacterial flagellar motor. An electrostatic model.

An electrostatic mechanism closely reproducing observed behavior in the bacterial flagellar motor.

Crystal structure of the cell wall anchor domain of MotB, a stator component of the bacterial flagellar motor: Implications for peptidoglycan recognition

Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor

Dynamics of the Bacterial Flagellar Motor: The Effects of Stator Compliance, Back Steps, Temperature, and Rotational Asymmetry

Electrostatic interactions between rotor and stator in the bacterial flagellar motor

Similar Documents

Structure and Function of the Bi-Directional Bacterial Flagellar Motor

Site-directed crosslinking identifies the stator-rotor interaction surfaces in a hybrid bacterial flagellar motor

Motility protein interactions in the bacterial flagellar motor.

Gate-controlled proton diffusion and protonation-induced ratchet motion in the stator of the bacterial flagellar motor

Torque and switching in the bacterial flagellar motor. An electrostatic model.

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