NDLI: Time to fatigue is increased in mouse muscle at 37°C; the role of iron and reactive oxygen species

Content Provider	PubMed Central
Author	Reardon, Trent F. Allen, David G.
Abstract	Studies exploring the rate of fatigue in isolated muscle at 37°C have produced mixed results. In the present study, muscle fibre bundles from the mouse foot were used to study the effect of temperature on the rate of muscle fatigue. Provided iron was excluded from the solutions, time to fatigue at 37°C was increased compared to 22°C (125 ± 8% of 22°C fatigue time). In contrast, when iron was present (∼1 μm), fatigue was accelerated (68 ± 10%). Iron can increase reactive oxygen species (ROS), which are believed to accelerate fatigue. The addition of 25–100 μm H2O2 at 22°C reduced time to fatigue to 80–20% of the control, respectively. Iron was added to cultured primary skeletal muscle cells to determine if iron could increase ROS production. Neither iron entry nor ROS production were detected in non-contracting muscle cells. The addition of 8-hydroxyquinoline, which facilitates iron entry, to iron–ascorbic acid solutions caused a rapid rise in intracellular iron and ROS. Our results indicate that time to fatigue in vitro is increased at 37°C relative to 22°C, but the addition of ROS can accelerate fatigue. An increase in muscle iron can accelerate ROS production, which may be important during or following exercise and in haemochromatosis, disuse atrophy and sarcopenia.
Related Links	http://dx.doi.org/10.1113/jphysiol.2009.173005
Ending Page	4716
Page Count	12
Starting Page	4705
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 19
Volume Number	587
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2009-10-01
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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