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| Content Provider | PubMed Central |
|---|---|
| Author | Brent, Jonathan Werner, Kristen Mccabe, Brian D. |
| Copyright Year | 2009 |
| Abstract | The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use of the Drosophila motor system is due to its high accessibility. It can be analyzed with single-cell resolution. There are 30 muscles per hemisegment whose arrangement within the peripheral body wall are known. A total of 31 motor neurons attach to these muscles in a pattern that has high fidelity. Using molecular biology and genetics, one can create transgenic animals or mutants. Then, one can study the developmental consequences on the morphology and function of the NMJ. Immunohistochemistry can be used to clearly image the components of the NMJ. In this article, we demonstrate how to use antibody staining to visualize the Drosophila larval NMJ. |
| Related Links | http://dx.doi.org/10.3791/1108 |
| Starting Page | 1108 |
| File Format | |
| ISSN | 1940087X |
| e-ISSN | 1940087X |
| Journal | Journal of Visualized Experiments : JoVE |
| Issue Number | 25 |
| Language | English |
| Publisher | MyJove Corporation |
| Publisher Date | 2009-03-01 |
| Access Restriction | Open |
| Rights Holder | MyJove Corporation |
| Subject Keyword | Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Chemical Engineering(all) Neuroscience(all) Medicine(all) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Immunology and Microbiology Biochemistry, Genetics and Molecular Biology Chemical Engineering |
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