NDLI: Cotranslational assembly of the yeast SET1C histone methyltransferase complex

Content Provider	PubMed Central
Author	Halbach, André Zhang, Haidi Wengi, Agnieszka Jablonska, Zofia Gruber, Isabel M. L. Halbeisen, Regula E. Dehé, Pierre-marie Patrick, Kemmeren Holstege, Frank Vincent, Géli Gerber, André P. Dichtl, Bernhard
Copyright Year	2009
Abstract	While probing the role of RNA for the function of SET1C/COMPASS histone methyltransferase, we identified SET1RC (SET1 mRNA-associated complex), a complex that contains SET1 mRNA and Set1, Swd1, Spp1 and Shg1, four of the eight polypeptides that constitute SET1C. Characterization of SET1RC showed that SET1 mRNA binding did not require associated Swd1, Spp1 and Shg1 proteins or RNA recognition motifs present in Set1. RNA binding was not observed when Set1 protein and SET1 mRNA were derived from independent genes or when SET1 transcripts were restricted to the nucleus. Importantly, the protein–RNA interaction was sensitive to EDTA, to the translation elongation inhibitor puromycin and to the inhibition of translation initiation in prt1-1 mutants. Taken together, our results support the idea that SET1 mRNA binding was dependent on translation and that SET1RC assembled on nascent Set1 in a cotranslational manner. Moreover, we show that cellular accumulation of Set1 is limited by the availability of certain SET1C components, such as Swd1 and Swd3, and suggest that cotranslational protein interactions may exert an effect in the protection of nascent Set1 from degradation.
Related Links	http://dx.doi.org/10.1038/emboj.2009.240
Ending Page	2970
Page Count	12
Starting Page	2959
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	19
Volume Number	28
Language	English
Publisher	Nature Publishing Group
Publisher Date	2009-10-07
Access Restriction	Open
Rights Holder	Nature Publishing Group
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Molecular Biology

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