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| Content Provider | PubMed Central |
|---|---|
| Author | Pourcel, Christine Hormigos, Katia Onteniente, Lucie Olga, Sakwinska Deurenberg, Ruud H. Vergnaud, Gilles |
| Abstract | We describe an improved multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme for genotyping Staphylococcus aureus. We compare its performance to those of multilocus sequence typing (MLST) and spa typing in a survey of 309 strains. This collection includes 87 epidemic methicillin-resistant S. aureus (MRSA) strains of the Harmony collection, 75 clinical strains representing the major MLST clonal complexes (CCs) (50 methicillin-sensitive S. aureus [MSSA] and 25 MRSA), 135 nasal carriage strains (133 MSSA and 2 MRSA), and 13 published S. aureus genome sequences. The results show excellent concordance between the techniques' results and demonstrate that the discriminatory power of MLVA is higher than those of both MLST and spa typing. Two hundred forty-two genotypes are discriminated with 14 VNTR loci (diversity index, 0.9965; 95% confidence interval, 0.9947 to 0.9984). Using a cutoff value of 45%, 21 clusters are observed, corresponding to the CCs previously defined by MLST. The variability of the different tandem repeats allows epidemiological studies, as well as follow-up of the evolution of CCs and the identification of potential ancestors. The 14 loci can conveniently be analyzed in two steps, based upon a first-line simplified assay comprising a subset of 10 loci (panel 1) and a second subset of 4 loci (panel 2) that provides higher resolution when needed. In conclusion, the MLVA scheme proposed here, in combination with available on-line genotyping databases (including http://mlva.u-psud.fr/), multiplexing, and automatic sizing, can provide a basis for almost-real-time large-scale population monitoring of S. aureus. |
| Related Links | http://dx.doi.org/10.1128/jcm.00267-09 |
| Ending Page | 3128 |
| Page Count | 8 |
| Starting Page | 3121 |
| File Format | |
| ISSN | 00951137 |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 10 |
| Volume Number | 47 |
| Language | English |
| Publisher | American Society for Microbiology (ASM) |
| Publisher Date | 2009-10-01 |
| Access Restriction | Open |
| Rights Holder | American Society for Microbiology (ASM) |
| Subject Keyword | Microbiology (medical) Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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