NDLI: The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice

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The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice

PHOSPHORYLATION OF CARDIAC TROPONIN I BY MAMMALIAN STERILE20-LIKE KINASE 1

NETROPSIN IMPROVES SURVIVAL FROM ENDOTOXEMIA BY DISRUPTING HMGA1-BINDING TO THE NOS2 PROMOTER

A common African polymorphism abolishes tyrosine sulfation of human anionic trypsinogen (PRSS2)

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The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice

Content Provider	PubMed Central
Author	Carlson, Bradley A. Schweizer, Ulrich Perella, Christine Shrimali, Rajeev K. Feigenbaum, Lionel Shen, Liya Speransky, Svetlana Floss, Thomas Jeong, Soon-jeong Watts, Jennifer Hoffmann, Victoria Combs, Gerald F. Gladyshev, Vadim N. Hatfield, Dolph
Copyright Year	2009
Abstract	STAF [Sec (selenocysteine) tRNA gene transcription activating factor] is a transcription activating factor for a number of RNA Pol III- and RNA Pol II-dependent genes including the Trsp [Sec tRNA gene], which in turn controls the expression of all selenoproteins. Here, the role of STAF in regulating expression of Sec tRNA and selenoproteins was examined. We generated transgenic mice expressing the Trsp transgene lacking the STAF-binding site and made these mice dependent on the transgene for survival by removing the wild-type Trsp. The level of Sec tRNA was unaffected or slightly elevated in heart and testis, but reduced ∼60% in liver and kidney, ∼70% in lung and spleen and ∼80% in brain and muscle compared with the corresponding organs in control mice. Moreover, the ratio of the two isoforms of Sec tRNA that differ by methylation at position 34 (Um34) was altered significantly, and the Um34-containing form was substantially reduced in all tissues examined. Selenoprotein expression in these animals was most affected in tissues in which the Sec tRNA levels were most severely reduced. Importantly, mice had a neurological phenotype strikingly similar to that of mice in which the selenoprotein P gene had been removed and their life span was substantially reduced. The results indicate that STAF influences selenoprotein expression by enhancing Trsp synthesis in an organ-specific manner and by controlling Sec tRNA modification in each tissue examined.
Related Links	http://dx.doi.org/10.1042/bj20081304
Ending Page	71
Page Count	11
Starting Page	61
File Format	PDF
ISSN	02646021
e-ISSN	14708728
Journal	The Biochemical journal
Issue Number	1
Volume Number	418
Language	English
Publisher Date	2009-02-15
Access Restriction	Open
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry

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The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice

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The selenocysteine tRNA STAF-binding region is essential for adequate selenocysteine tRNA status, selenoprotein expression and early age survival of mice