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| Content Provider | PubMed Central |
|---|---|
| Author | Hendrickson, Howard Elizabeth, Laurenzana Owens, S. Michael |
| Abstract | High-throughput liquid chromatography with tandem mass spectrometric detection (LC-MS/MS) methodology for the determination of methamphetamine (METH), amphetamine (AMP), 4-hydroxymethamphetamine (4-OH-METH), and 4-hydroxyamphetamine (4-OH-AMP) was developed and validated using simple trichloroacetic acid sample treatment. The method was validated in rat serum, brain, and testis. Lower limits-of-quantitation (LOQ) for METH and AMP were 1 ng·mL−1 using positive ion electrospray tandem mass spectrometry (MS/MS). The accuracy of the method was within 25% of the actual values over a wide range of analyte concentrations. The within-assay precision was better than 12% (coefficient of variation). The method was linear over a wide dynamic range (0.3–1000 ng·mL−1). Quantitation was possible in all 3 matrices using only serum standards because of minimal matrix-associated ion effects or the use of an internal standard. Finally, the LC-MS/MS method was used to determine serum, brain, and testis METH and AMP concentrations during a subcutaneous infusion (5.6 mg kg−1 day−1) of METH in rats. Concentrations of 4-OH-AMP and 4-OH-METH were below the LOQ in experimental samples. The bias introduced by using serum calibrators for the determination of METH and AMP concentrations in testis and brain was less than 8% and insignificant relative to the interanimal variability. |
| Related Links | http://dx.doi.org/10.1208/aapsj080480 |
| Starting Page | 709 |
| File Format | |
| ISSN | 15507416 |
| e-ISSN | 15507416 |
| Journal | The AAPS Journal |
| Issue Number | 4 |
| Volume Number | 8 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2006-12-01 |
| Access Restriction | Open |
| Rights Holder | Springer-Verlag |
| Subject Keyword | Pharmaceutical Science Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmaceutical Science |
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