NDLI: The unitary event underlying multiquantal excitatory postsynaptic currents at a hair cell's ribbon synapse

Content Provider	PubMed Central
Author	Li, Geng-lin Keen, Erica Daniel, Andor-ardó Hudspeth, A. J. Gersdorff, Von Henrique
Abstract	Excitatory postsynaptic currents (EPSCs) at the synapses of sensory receptors and of some central-nervous-system neurons include large events thought to represent the synchronous release of the neurotransmitter contained in several synaptic vesicles in a process known as multiquantal release. However, determination of the unitary, quantal size underlying such putatively multiquantal events has proven difficult at hair-cell synapses, hindering confirmation that large EPSCs are in fact multiquantal. Here we address this issue by performing presynaptic membrane capacitance measurements together with paired recordings at the ribbon synapses of adult hair cells. These simultaneous pre- and postsynaptic assays of exocytosis, together with electron-microscopic estimates of single-vesicle capacitance, allow us to estimate a single-vesicle EPSC charge of about −45 fC, a value in close agreement with the mean postsynaptic charge transfer of uniformly small EPSCs recorded during periods of presynaptic hyperpolarization. By thus establishing the magnitude of the fundamental quantal event at this peripheral sensory synapse, we provide evidence that the majority of spontaneous and evoked EPSCs are multiquantal. Furthermore, we show that the prevalence of uniquantal versus multiquantal events is Ca2+-dependent. Paired recordings also reveal a tight correlation between membrane capacitance increase and evoked EPSC charge, indicating that glutamate release during prolonged hair-cell depolarization does not significantly saturate or desensitize postsynaptic AMPA receptors. We propose that the large EPSCs reflect the highly synchronized release of multiple vesicles at single presynaptic active zones through a compound or coordinated vesicle fusion mechanism.
Related Links	http://dx.doi.org/10.1523/jneurosci.0514-09.2009
Ending Page	7568
Page Count	11
Starting Page	7558
File Format	PDF
ISSN	02706474
e-ISSN	15292401
Journal	The Journal of neuroscience : the official journal of the Society for Neuroscience
Issue Number	23
Volume Number	29
Language	English
Publisher Date	2009-06-10
Access Restriction	Open
Subject Keyword	Neuroscience(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Phase-locking precision is enhanced by multiquantal release at an auditory hair cell ribbon synapse

Molecular Anatomy of the Hair Cell's Ribbon Synapse

Two modes of release shape the postsynaptic response at the inner hair cell ribbon synapse

Dendritic HCN Channels Shape Excitatory Postsynaptic Potentials at the Inner Hair Cell Afferent Synapse in the Mammalian Cochlea

Time and intensity coding at the hair cell's ribbon synapse

Quantal components of the excitatory postsynaptic currents at a rat central auditory synapse

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Short-term facilitation modulates size and timing of the synaptic response at the inner hair cell ribbon synapse

Passive Diffusion as a Mechanism Underlying Ribbon Synapse Vesicle Release and Resupply

The unitary event underlying multiquantal excitatory postsynaptic currents at a hair cell's ribbon synapse

Similar Documents

Phase-locking precision is enhanced by multiquantal release at an auditory hair cell ribbon synapse

Molecular Anatomy of the Hair Cell's Ribbon Synapse

Two modes of release shape the postsynaptic response at the inner hair cell ribbon synapse

Dendritic HCN Channels Shape Excitatory Postsynaptic Potentials at the Inner Hair Cell Afferent Synapse in the Mammalian Cochlea

Time and intensity coding at the hair cell's ribbon synapse

Quantal components of the excitatory postsynaptic currents at a rat central auditory synapse

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Short-term facilitation modulates size and timing of the synaptic response at the inner hair cell ribbon synapse

Passive Diffusion as a Mechanism Underlying Ribbon Synapse Vesicle Release and Resupply

The unitary event underlying multiquantal excitatory postsynaptic currents at a hair cell's ribbon synapse