NDLI: Development of a simple and rapid elisa of urinary cotinine for epidemiological application

Please wait, while we are loading the content...

Smoking conditions and the relationships between smoking habits and such factors as exercise habits and morning diet among male students aged 16 to 20 years

An investigation into the effects of vacations on the health status in male white-collar workers

Food intake and food consumption patterns of hospital workers in the dominican republic

Survey on negative impact of chironomid midges (Diptera) on bronchial asthmatic patients in a hyper-eutrophic lake area in Japan

Prevalence of mood disorders according to dsm-iii-r criteria in the community elderly residents in Japan

Modifying effects of maharishi amrit kalash 4 and 5 on phagocytic and digestive functions of macrophages in male icr mice

Do radiofrequency radiation affect the auditory system of people with occupational exposure?

Reproducibility of an interview questionnaire on sexual behavior in japanese middle-aged or elderly males

Erratum

Year: 1998, Volume: 2

Year: 1997, Volume: 2

Year: 1997, Volume: 1

Development of a simple and rapid elisa of urinary cotinine for epidemiological application

Content Provider	PubMed Central
Author	Yoshioka, Nobuo Yoshiko, Dohi Yonemasu, Kunio
Abstract	Highly stereospecific polyclonal antibodies (anti-CN) to cotinine (CN), a major metabolite of nicotine, were prepared from rabbit antisera to CN-linked keyhole limpet hemocyanin (KLH) by removing the antibodies to KLH and to its binding regions of CN. This was achieved by using immunoadsorbents consisting of insolubilized KLH onto CNBr-activated-Sepharose 4B. A new simple and rapid enzyme-linked immunosorbent assay (ELISA) of urinary CN was developed using the anti-CN. A brief outline of the method is as follows: CN-bovine thyroglobulin complex is coated onto wells of microtiter plates (1 ng/well), and then aliquots of urine samples or standard CN solutions were added followed by appropriate dilution of the anti-CN. The bound anti-CN antibodies are quantified spectrophotometrically with horseradish peroxidase-labelled anti-rabbit IgG and 2,2′-azino-di (3-ethylbenz- thiazoline)-6-sulfonic acid. Measurement of CN concentration in urine samples can be read off on a calibration curve drawn by using standard CN solutions. The standard curve ranged from 1 ng to 4 μ g /ml with an estimated lower limit of sensitivity of 7–8 ng/ml, resulting in within/between-assay CV (coefficient of variation) of lower than 10%. The method allowed one to assay more than 40 samples in duplicate by using just one plate, and is thus easily applicable to epidemiological investigations into exposure status to tobacco smoke.
Related Links	http://dx.doi.org/10.1007/bf02931233
Ending Page	16
Page Count	5
Starting Page	12
File Format	PDF
ISSN	1342078X
e-ISSN	13474715
Journal	Environmental Health and Preventive Medicine
Issue Number	1
Volume Number	3
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-04-01
Access Restriction	Open
Rights Holder	Springer-Verlag
Subject Keyword	Public Health, Environmental and Occupational Health Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Active and passive exposure status to tobacco smoke of department store employees measured by cotinine ELISA

Salivary cotinine concentrations in daily smokers in Barcelona, Spain: a cross-sectional study

ELISA—a simple test for detecting and differentiating antibodies to closely related orthopoxviruses

Simultaneous measurement of urinary total nicotine and cotinine as biomarkers of active and passive smoking among Japanese individuals

Respiratory Symptoms and Urinary Cotinine Levels in Pre-school Children Exposed to Environmental Tobacco Smoke

Cigarette Rod Length and Its Impact on Serum Cotinine and Urinary Total NNAL Levels, NHANES 2007–2010

Urinary Cadmium as a Marker of Exposure in Epidemiological Studies

Estimation and projection of adult AIDS cases: a simple epidemiological model.

A simple strip test for the diagnosis of urinary tract infection in general practice.

Development of a simple and rapid elisa of urinary cotinine for epidemiological application

Similar Documents

Active and passive exposure status to tobacco smoke of department store employees measured by cotinine ELISA

Salivary cotinine concentrations in daily smokers in Barcelona, Spain: a cross-sectional study

ELISA—a simple test for detecting and differentiating antibodies to closely related orthopoxviruses

Simultaneous measurement of urinary total nicotine and cotinine as biomarkers of active and passive smoking among Japanese individuals

Respiratory Symptoms and Urinary Cotinine Levels in Pre-school Children Exposed to Environmental Tobacco Smoke

Cigarette Rod Length and Its Impact on Serum Cotinine and Urinary Total NNAL Levels, NHANES 2007–2010

Urinary Cadmium as a Marker of Exposure in Epidemiological Studies

Estimation and projection of adult AIDS cases: a simple epidemiological model.

A simple strip test for the diagnosis of urinary tract infection in general practice.

Development of a simple and rapid elisa of urinary cotinine for epidemiological application