NDLI: CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation*

Content Provider	PubMed Central
Author	Nojiri, Mari Loyet, Kelly M. Klenchin, Vadim A. Gregory, Kabachinski Martin, Thomas F. J.
Abstract	CAPS (Ca2+-dependent activator protein for secretion) functions in priming Ca2+-dependent vesicle exocytosis, but the regulation of CAPS activity has not been characterized. Here we show that phosphorylation by protein kinase CK2 is required for CAPS activity. Dephosphorylation eliminated CAPS activity in reconstituting Ca2+-dependent vesicle exocytosis in permeable and intact PC12 cells. Ser-5, -6, and -7 and Ser-1281 were identified by mass spectrometry as the major phosphorylation sites in the 1289 residue protein. Ser-5, -6, and -7 but not Ser-1281 to Ala substitutions abolished CAPS activity. Protein kinase CK2 phosphorylated CAPS in vitro at these sites and restored the activity of dephosphorylated CAPS. CK2 is the likely in vivo CAPS protein kinase based on inhibition of phosphorylation by tetrabromo-2-benzotriazole in PC12 cells and by the identity of in vivo and in vitro phosphorylation sites. CAPS phosphorylation by CK2 was constitutive, but the elevation of Ca2+ in synaptosomes increased CAPS Ser-5 and -6 dephosphorylation, which terminates CAPS activity. These results identify a functionally important N-terminal phosphorylation site that regulates CAPS activity in priming vesicle exocytosis.
Related Links	http://dx.doi.org/10.1074/jbc.m109.017483
Ending Page	18714
Page Count	8
Starting Page	18707
File Format	PDF
ISSN	00219258
e-ISSN	1083351X
Journal	The Journal of Biological Chemistry
Issue Number	28
Volume Number	284
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-07-10
Access Restriction	Open
Rights Holder	American Society for Biochemistry and Molecular Biology
Subject Keyword	Cell Biology Biochemistry Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation

CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation

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CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation*

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CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation

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CAPS Activity in Priming Vesicle Exocytosis Requires CK2 Phosphorylation*