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| Content Provider | PubMed Central |
|---|---|
| Author | Sandin, R. L. Knapp, C. C. Hall, G. S. Washington, J. A. Rutherford, I. |
| Abstract | The value and convenience of testing for specific anti-Toxoplasma gondii antibodies have led to the development of various antibody detection methods, such as the enzyme immunoassay. Two enzyme immunoassays, the Vitek Immuno-Diagnostic Assay System (VIDAS; Vitek Systems, Hazelwood, Mo.) and the Toxostat Test Kit (TST; Whittaker Bioproducts, Walkersville, Md.), were compared for their ability to detect T. gondii immunoglobulin G antibodies in fresh human sera. Specimens were tested according to the instructions of each manufacturer. Of 304 serum specimens tested, the results for 282 (93%) agreed in both assays (77 were positive and 205 were negative). The results for the remaining 22 (7%) specimens were discrepant; 20 of the 22 specimens tested low positive or equivocal by Toxostat and negative by VIDAS. Upon retesting, 8 of these 22 specimens were in concordance, increasing the level of agreement to 95.3%. Intra-assay reproducibility was tested with four to nine replicates of each of seven samples (four positive, one negative, and two equivocal) by both tests and six replicates of one standard by VIDAS. Coefficients of variation (CVs) for VIDAS were 6, 8, 10, 15, 18, 19, and 23% for the samples and 14% for the standard. For Toxostat the CVs were 2, 8, 10, 10, 13, and 16%. In general, CVs for VIDAS were slightly larger than the CVs for Toxostat. Day-to-day variability over 3 days was tested by VIDAS for only six samples (three positive, two negative, and one equivocal) and four standards; CVs ranged from 2 to 15% for the samples and from 6 to 13% for the standards. In conclusions, VIDAS is a rapid, convenient, non-labor-intensive, and easily performed test for the detection of immunoglobulin G antibodies to T. gondii in serum specimens. |
| Starting Page | 2763 |
| File Format | |
| ISSN | 1098660X |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 12 |
| Volume Number | 29 |
| Language | English |
| Publisher Date | 1991-12-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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