NDLI: Inter-sarcomere coordination in muscle revealed through individual sarcomere response to quick stretch

Content Provider	PubMed Central
Author	Shimamoto, Yuta Suzuki, Madoka Mikhailenko, Sergey V. Yasuda, Kenji Ishiwata, Shin'ichi
Abstract	The force generation and motion of muscle are produced by the collective work of thousands of sarcomeres, the basic structural units of striated muscle. Based on their series connection to form a myofibril, it is expected that sarcomeres are mechanically and/or structurally coupled to each other. However, the behavior of individual sarcomeres and the coupling dynamics between sarcomeres remain elusive, because muscle mechanics has so far been investigated mainly by analyzing the averaged behavior of thousands of sarcomeres in muscle fibers. In this study, we directly measured the length-responses of individual sarcomeres to quick stretch at partial activation, using micromanipulation of skeletal myofibrils under a phase-contrast microscope. The experiments were performed at ADP-activation (1 mM MgATP and 2 mM MgADP in the absence of Ca2+) and also at Ca2+-activation (1 mM MgATP at pCa 6.3) conditions. We show that under these activation conditions, sarcomeres exhibit 2 distinct types of responses, either “resisting” or “yielding,” which are clearly distinguished by the lengthening distance of single sarcomeres in response to stretch. These 2 types of sarcomeres tended to coexist within the myofibril, and the sarcomere “yielding” occurred in clusters composed of several adjacent sarcomeres. The labeling of Z-line with anti-α-actinin antibody significantly suppressed the clustered sarcomere “yielding.” These results strongly suggest that the contractile system of muscle possesses the mechanism of structure-based inter-sarcomere coordination.
Related Links	http://dx.doi.org/10.1073/pnas.0813288106
Ending Page	11959
Page Count	6
Starting Page	11954
File Format	PDF
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	29
Volume Number	106
Language	English
Publisher	National Academy of Sciences
Publisher Date	2009-07-21
Access Restriction	Open
Rights Holder	National Academy of Sciences
Subject Keyword	General Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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