Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | PubMed Central |
|---|---|
| Author | Henriksen, A. Z. Maeland, J. A. |
| Abstract | Antibodies to Escherichia coli outer membrane proteins in sera from healthy persons and from patients bacteremic with various enteric or nonenteric bacteria were measured by an enzyme-linked immunosorbent assay (ELISA). Outer membranes were prepared from E. coli O55. Serum was absorbed with E. coli O55 lipopolysaccharide and diluted 1:100 for immunoglobulin A (IgA) or IgM and 1:1,000 for IgG antibodies. Paired serum specimens were obtained from the 56 patients included in the study (the first specimen on the day of positive blood culture and the second specimen 8 to 12 days later) and compared with sera from blood donors (n = 50) as controls. On an average, the patients bacteremic with enterobacteria (n = 40) showed increased levels of antibodies of all three immunoglobulin classes in the first serum specimens and significantly higher levels in the second specimens compared with the controls, although with considerable case-to-case variation. Increased levels of IgG antibodies showed the best combination of diagnostic specificity (100%) and sensitivity (53%) for bacteremia caused by enteric bacilli. Mostly, the antibody response was directed against the major E. coli O55 outer membrane proteins at 81,000, 38,500, 33,500, and 7,500 molecular weights as shown by Western blot (immunoblot) analysis. Some of the patients bacteremic with nonenteric bacteria showed increased levels of IgA antibodies, but not of IgG or IgM antibodies. Cross-reactivity of the nonenteric blood culture isolates with the E. coli outer membrane preparation was not demonstrated. The cross-reactivity of the E. coli O55 outer membrane proteins with those of enteric bacilli of other genera was examined by absorption experiments. Western blots with serum absorbed with live E. coli O55 provided evidence that the epitopes of the outer membrane protein at 7,500 molecular weight were available for antibody binding at the bacterial surface, and that at least some of the epitopes of the 38,500- and 33,500-molecular -weight proteins were accessible to antibodies. The results suggest that an ELISA for the measurement of antibodies against cross-reactive outer membrane proteins from enteric bacilli may be useful in the diagnosis of serious infections caused by members of the family Enterobacteriaceae, and that antibodies to the major outer membrane proteins may have an immunobiological function. |
| Starting Page | 2181 |
| File Format | |
| ISSN | 1098660X |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 11 |
| Volume Number | 25 |
| Language | English |
| Publisher Date | 1987-11-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|