NDLI: Functional and computational studies of the ligand-associated metal binding site of β3 integrins

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Refinement of non-calorimetric determination of the change in heat capacity, ΔCp, of protein unfolding and validation across a wide temperature range

Functional and computational studies of the ligand-associated metal binding site of β3 integrins

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Functional and computational studies of the ligand-associated metal binding site of β3 integrins

Content Provider	PubMed Central
Author	Murcia, Marta Jirouskova, Marketa Li, Jihong Coller, Barry S. Filizola, Marta
Abstract	A combination of experimental and computational approaches was used to provide a structural context for the role of the β3 integrin subunit ligand-associated metal binding site (LIMBS) in the binding of physiological ligands to β3 integrins. Specifically, we have carried out (1) adhesion assays on cells expressing normal αIIbβ3, normal αVβ3, or the corresponding β3 D217A LIMBS mutants; and (2) equilibrium and nonequilibrium (steered) molecular dynamics (MD) simulations of eptifibatide in complex with either a fully hydrated normal αIIbβ3 integrin fragment (αIIb β-propeller and the β3 βA (I-like), hybrid, and PSI domains) or the equivalent β3 D217A mutant. Normal αIIbβ3 expressing cells adhered to immobilized fibrinogen and echistatin, whereas cells expressing the αIIbβ3 D217A LIMBS mutant failed to adhere to either ligand. Similarly, the equivalent αVβ3 mutant was unable to support adhesion to vitronectin or fibrinogen. The αIIbβ3 D217A mutation increased the binding of mAb AP5, which recognizes a ligand-induced binding site (LIBS) in the β3 PSI domain, indicating that this mutation induced allosteric changes in the protein. Steered MD simulating the unbinding of eptifibatide from either normal αIIbβ3 or the equivalent β3 D217A mutant suggested that the reduction in ligand binding caused by the LIMBS mutant required the loss of both the LIMBS and the metal ion-dependent adhesion site (MIDAS) metal ions. Our computational results indicate that the LIMBS plays a crucial role in ligand binding to αIIbβ3 by virtue of its effects on the coordination of the MIDAS.
Related Links	http://dx.doi.org/10.1002/prot.21859
Starting Page	1779
File Format	PDF
ISSN	10970134
e-ISSN	10970134
Journal	Proteins
Issue Number	4
Volume Number	71
Language	English
Publisher Date	2008-06-01
Access Restriction	Open
Subject Keyword	Proteomics Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biochemistry Molecular Biology

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