NDLI: CIA2 Coordinately Up-Regulates Protein Import and Synthesis in Leaf Chloroplasts1 [W] [OA]

Content Provider	PubMed Central
Author	Sun, Chih-wen Huang, Yen-chiao Chang, Hsin-yen
Abstract	Plastid biogenesis and maintenance depend on the coordinated assembly of proteins imported from the cytosol with proteins translated within plastids. Chloroplasts in leaf cells have a greater need for protein import and protein synthesis than plastids in other organs due to the large amount of proteins required for photosynthesis. We previously reported that the Arabidopsis (Arabidopsis thaliana) transcription factor CIA2 specifically up-regulates leaf expression of genes encoding protein translocons Toc33 and Toc75, which are essential for protein import into chloroplasts. Protein import efficiency was therefore reduced in cia2 mutant chloroplasts. To further understand the function of CIA2, gene expression profiles of the wild type and a cia2 mutant were compared by microarray analysis. Interestingly, in addition to genes encoding protein translocon components, other genes down-regulated in cia2 almost exclusively encode chloroplast ribosomal proteins. Isolated cia2 mutant chloroplasts showed reduced translation efficiency and steady-state accumulation of plastid-encoded proteins. When CIA2 was ectopically expressed in roots, expression of both the protein translocon and ribosomal protein genes increased. Further analyses in vivo revealed that CIA2 up-regulated these genes by binding directly to their promoter regions. We propose that CIA2 is an important factor responsible for fulfilling the higher protein demands of leaf chloroplasts by coordinately increasing both protein import and protein translation efficiencies.
Related Links	http://dx.doi.org/10.1104/pp.109.137240
Ending Page	888
Page Count	10
Starting Page	879
File Format	PDF
ISSN	00320889
e-ISSN	15322548
Journal	Plant Physiology
Issue Number	2
Volume Number	150
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2009-06-01
Access Restriction	Open
Rights Holder	American Society of Plant Biologists
Subject Keyword	Plant Science Genetics Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Genetics Physiology Plant Science

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CIA2 Coordinately Up-Regulates Protein Import and Synthesis in Leaf Chloroplasts

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CIA2 Coordinately Up-Regulates Protein Import and Synthesis in Leaf Chloroplasts1 [W] [OA]

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