NDLI: Functional maturation of the exocytotic machinery at gerbil hair cell ribbon synapses

Content Provider	PubMed Central
Author	Johnson, Stuart L. Franz, Christoph Knipper, Marlies Marcotti, Walter
Copyright Year	2009
Abstract	Auditory afferent fibre activity in mammals relies on neurotransmission at hair cell ribbon synapses. Developmental changes in the Ca2+ sensitivity of the synaptic machinery allow inner hair cells (IHCs), the primary auditory receptors, to encode Ca2+ action potentials (APs) during pre-hearing stages and graded receptor potentials in adult animals. However, little is known about the time course of these changes or whether the kinetic properties of exocytosis differ as a function of IHC position along the immature cochlea. Furthermore, the role of afferent transmission in outer hair cells (OHCs) is not understood. Calcium currents and exocytosis (measured as membrane capacitance changes: ΔC m) were measured with whole-cell recordings from immature gerbil hair cells using near-physiological conditions. The kinetics, vesicle pool depletion and Ca2+ coupling of exocytosis were similar in apical and basal immature IHCs. This could indicate that possible differences in AP activity along the immature cochlea do not require synaptic specialization. Neurotransmission in IHCs became mature from postnatal day 20 (P20), although changes in its Ca2+ dependence occurred at P9–P12 in basal and P12–P15 in apical cells. OHCs showed a smaller ΔC m than IHCs that was reflected by fewer active zones in OHCs. Otoferlin, the proposed Ca2+ sensor in cochlear hair cells, was similarly distributed in both cell types despite the high-order exocytotic Ca2+ dependence in IHCs and the near-linear relation in OHCs. The results presented here provide a comprehensive study of the function and development of hair cell ribbon synapses.
Related Links	http://dx.doi.org/10.1113/jphysiol.2009.168542
Ending Page	1726
Page Count	12
Starting Page	1715
File Format	PDF
ISSN	00223751
e-ISSN	14697793
Journal	The Journal of Physiology
Issue Number	Pt 8
Volume Number	587
Language	English
Publisher	Blackwell Science Inc
Publisher Date	2009-04-15
Access Restriction	Open
Rights Holder	Blackwell Science Inc
Subject Keyword	Physiology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Physiology Sports Science

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