NDLI: A Vitamin B12-Based System for Conditional Expression Reveals dksA To Be an Essential Gene in Myxococcus xanthus ▿

Content Provider	PubMed Central
Author	García Moreno, Diana Polanco, María Carmen Gloria, Navarro-avilés Murillo, Francisco J. Padmanabhan, S. Montserrat, Elías-arnanz
Abstract	Myxococcus xanthus is a prokaryotic model system for the study of multicellular development and the response to blue light. The previous analyses of these processes and the characterization of new genes would benefit from a robust system for controlled gene expression, which has been elusive so far for this bacterium. Here, we describe a system for conditional expression of genes in M. xanthus based on our recent finding that vitamin B12 and CarH, a MerR-type transcriptional repressor, together downregulate a photoinducible promoter. Using this system, we confirmed that M. xanthus rpoN, encoding σ54, is an essential gene, as reported earlier. We then tested it with ftsZ and dksA. In most bacteria, ftsZ is vital due to its role in cell division, whereas null mutants of dksA, whose product regulates the stringent response via transcriptional control of rRNA and amino acid biosynthesis promoters, are viable but cause pleiotropic effects. As with rpoN, it was impossible to delete endogenous ftsZ or dksA in M. xanthus except in a merodiploid background carrying another functional copy, which indicates that these are essential genes. B12-based conditional expression of ftsZ was insufficient to provide the high intracellular FtsZ levels required. With dksA, as with rpoN, cells were viable under permissive but not restrictive conditions, and depletion of DksA or σ54 produced filamentous, aberrantly dividing cells. dksA thus joins rpoN in a growing list of genes dispensable in many bacteria but essential in M. xanthus.
Related Links	http://dx.doi.org/10.1128/JB.01737-08
Starting Page	3108
File Format	PDF
ISSN	10985530
e-ISSN	10985530
Journal	Journal of Bacteriology
Issue Number	9
Volume Number	191
Language	English
Publisher	American Society for Microbiology (ASM)
Publisher Date	2009-05-01
Access Restriction	Open
Rights Holder	American Society for Microbiology (ASM)
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Microbiology

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A Vitamin B12-Based System for Conditional Expression Reveals dksA To Be an Essential Gene in Myxococcus xanthus ▿

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