NDLI: Using Proteomic Approach to Identify Tumor-Associated Antigens as Markers in Hepatocellular Carcinoma

Content Provider	PubMed Central
Author	Looi, Kok Sun Nakayasu, Ernesto S. Diaz, Raquel A. De Tan, Eng M. Almeida, Igor C. Zhang, Jian-ying
Abstract	Many studies have demonstrated that intracellular proteins, which are involved in carcinogenesis, can provoke autoantibody responses. Therefore, autoantibodies can be used clinically for cancer detection and for proteomic analysis in identification of tumor-associated antigens (TAAs) that are potentially involved in malignant transformation. Liver cancer, especially hepatocellular carcinoma (HCC), is one of the most common tumors in the world. The majority of people with HCC will die within 1 year of its detection. This high case fatality rate can partially be attributed to a lack of diagnostic methods that allow early detection. In the present study, sera from 20 patients with HCC, 30 patients with chronic hepatitis (CH), and 30 patients with liver cirrhosis (LC) as well as sera from 10 normal individuals were used in a proteomic approach to identify HCC-related TAAs. Thirty-four immunoreactive protein spots were excised from the two-dimensional gel electrophoresis (2DE), digested with trypsin, and subsequently analyzed by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Of 34 immunoreactive protein spots, 28 were identified. Seventeen of them were not only reactive with serum antibodies in HCC but also with antibodies in pre-HCC conditions, and 11 were only reactive with serum antibodies in HCC but not with antibodies in pre-HCC conditions. In the subsequent analysis, two representative proteins, HSP60 and HSP70, were selected as examples for the validation purpose. The results from immunoassay were consistent with the data from proteomic analysis, supporting our hypothesis that proteins identified with autoantibodies that have been present in precancer conditions may be not appropriate to use as TAA markers in cancer detection.
Related Links	http://dx.doi.org/10.1021/pr800273h
Ending Page	4012
Page Count	9
Starting Page	4004
File Format	PDF
ISSN	15353893
e-ISSN	15353907
Journal	Journal of proteome research
Issue Number	9
Volume Number	7
Language	English
Publisher Date	2008-09-05
Access Restriction	Open
Subject Keyword	Biochemistry Chemistry(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Using Proteomic Approach to Identify Tumor-Associated Proteins as Biomarkers in Human Esophageal Squamous Cell Carcinoma

Proteomic-Based Approach for the Identification of Tumor Markers Associated with Hepatocellular Carcinoma

Proteomic-Based Approach for the Identification of Tumor Markers Associated with Hepatocellular Carcinoma

A Quantitative Proteomic Approach for Identification of Potential Biomarkers in Hepatocellular Carcinoma

Autoantibodies to tumor-associated antigens as biomarkers in human hepatocellular carcinoma (HCC)

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma

Autoantibodies to tumor-associated antigens as biomarkers in human hepatocellular carcinoma (HCC)

Identification of N-Glycan Serum Markers Associated with Hepatocellular Carcinoma from Mass Spectrometry Data

Using Serial Analysis of Gene Expression to Identify Tumor Markers and Antigens

Using Proteomic Approach to Identify Tumor-Associated Antigens as Markers in Hepatocellular Carcinoma

Similar Documents

Using Proteomic Approach to Identify Tumor-Associated Proteins as Biomarkers in Human Esophageal Squamous Cell Carcinoma

Proteomic-Based Approach for the Identification of Tumor Markers Associated with Hepatocellular Carcinoma

Proteomic-Based Approach for the Identification of Tumor Markers Associated with Hepatocellular Carcinoma

A Quantitative Proteomic Approach for Identification of Potential Biomarkers in Hepatocellular Carcinoma

Autoantibodies to tumor-associated antigens as biomarkers in human hepatocellular carcinoma (HCC)

Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma

Autoantibodies to tumor-associated antigens as biomarkers in human hepatocellular carcinoma (HCC)

Identification of N-Glycan Serum Markers Associated with Hepatocellular Carcinoma from Mass Spectrometry Data

Using Serial Analysis of Gene Expression to Identify Tumor Markers and Antigens

Using Proteomic Approach to Identify Tumor-Associated Antigens as Markers in Hepatocellular Carcinoma