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| Content Provider | PubMed Central |
|---|---|
| Author | Theil, K. W. Mccloskey, C. M. |
| Abstract | The genome electropherotyping technique was used to examine group A rotaviral infections of diarrheic calves ranging from 1 to 85 days of age in 2 beef and 27 dairy herds. Coelectrophoresis studies demonstrated 38 distinct bovine group A rotavirus genome electropherotypes; all were long genome electropherotypes, and none had extra segments or unusual segment rearrangements. Genome electropherotypes in fecal specimens from diarrheic calves previously inoculated orally with a commercial, modified-live group A rotavirus vaccine differed from the vaccine genome electropherotype. Generally, when fecal specimens for genome electropherotyping were collected from two or more different calves within the same herd over a relatively short time, only one genome electropherotype was detected within a given herd. Different genome electropherotypes were detected in the same herd, however, when fecal specimens were obtained from different diarrheic calves over longer intervals (6 months or more). Twenty-three group A rotavirus strains with distinct genome electropherotypes, from diarrheic calves in 22 herds, were isolated and plaque purified in cell culture, and all were subgroup 1 group A rotaviruses. Non-group A rotavirus genome electropherotypes were not detected in 131 fecal specimens, negative for group A rotavirus, collected from diarrheic calves in 17 dairy herds. |
| Starting Page | 126 |
| File Format | |
| ISSN | 1098660X |
| e-ISSN | 1098660X |
| Journal | Journal of Clinical Microbiology |
| Issue Number | 1 |
| Volume Number | 27 |
| Language | English |
| Publisher Date | 1989-01-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology (medical) |
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