NDLI: Herpes simplex virus detection by macroscopic reading after overnight incubation and immunoperoxidase staining.

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Bacteriological findings of acute maxillary sinusitis in young adults.

Macroscopic purulence, leukocyte counts, and bacterial morphotypes in relation to culture findings for sinus secretions in acute maxillary sinusitis.

Rapid chemiluminescent nucleic acid assays for detection of TEM-1 beta-lactamase-mediated penicillin resistance in Neisseria gonorrhoeae and other bacteria.

Characteristics of coagulase-negative staphylococci that help differentiate these species and other members of the family Micrococcaceae.

Biotyping coagulase-negative staphylococci.

Simultaneous detection of antibodies to cytomegalovirus and herpes simplex virus by using flow cytometry and a microsphere-based fluorescence immunoassay.

Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody.

Monoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli.

Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains.

Enzyme-linked immunosorbent assay for diagnosis of chronic Q fever.

Isolation and characterization of mutant strains of Bordetella bronchiseptica lacking dermonecrotic toxin-producing ability.

Meningococcal phenotypic and genotypic characteristics and human antibody levels.

Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA-DNA hybridization and phenotype.

Detection of human calicivirus antigen and antibody by enzyme-linked immunosorbent assays.

Characterization of Escherichia coli serotype O157:H7.

Herpes simplex virus detection by macroscopic reading after overnight incubation and immunoperoxidase staining.

Vibrio cholerae non-O1: production of cell-associated hemagglutinins and in vitro adherence to mucus coat and epithelial surfaces of the villi and lymphoid follicles of human small intestines treated with formalin.

Microtechnique for serum opacity factor characterization of group A streptococci adaptable to the use of human sera.

Detection of cytomegalovirus from blood leukocytes separated by sepracell-MN and Ficoll-Paque/Macrodex methods.

Determination of antibody response to influenza virus surface glycoproteins by kinetic enzyme-linked immunosorbent assay.

Evaluation of Abbott CMV-M enzyme immunoassay for detection of cytomegalovirus immunoglobulin M antibody.

Evaluation of different fixatives and treatments for immunohistochemical demonstration of Coxiella burnetti in paraffin-embedded tissues.

Identification of a 17-kilodalton Fasciola hepatica immunodiagnostic antigen by the enzyme-linked immunoelectrotransfer blot technique.

Comparison of the Quantum II, API Yeast Ident, and AutoMicrobic systems for identification of clinical yeast isolates.

Immunologic diversity among serogroup 1 Legionella pneumophila urinary antigens demonstrated by monoclonal antibody enzyme-linked immunosorbent assays.

Vancomycin-resistant gram-positive bacteria isolated from human sources.

Determination of serum bactericidal activity against Escherichia coli by an automated photometric method.

Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of Bordetella pertussis filamentous hemagglutinin.

Plasmid analysis of Shigella dysenteriae type 1 isolates obtained from widely scattered geographical locations.

Immunoblot analysis of serologic response to outer membrane proteins of Escherichia coli in elderly individuals with urinary tract infections.

Relative frequencies of rotavirus serotypes 1, 2, 3, and 4 in Venezuelan infants with gastroenteritis.

Comparative evaluation of selective media for isolation of Pseudomonas cepacia from cystic fibrosis patients and environmental sources.

Immunoglobulin A antibodies to Trypanosoma cruzi antigens in digestive forms of Chagas' disease.

Oral infections and septicemia in immunocompromised patients with hematologic malignancies.

Dermatophyte-hormone relationships: characterization of progesterone-binding specificity and growth inhibition in the genera Trichophyton and Microsporum.

Latex agglutination and enzyme-linked immunosorbent assays for cytomegalovirus serologic screening of transplant donors and recipients.

Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex.

Evaluation of [3H]thymidine uptake method for studying growth of spiroplasmas under various conditions.

Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay.

DNA probe technology for rapid detection of Haemophilus influenzae in clinical specimens.

Isolation of a bovine rotavirus with a "super-short" RNA electrophoretic pattern from a calf with diarrhea.

Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.

Enzymatic degradation of urinary indoxyl sulfate by Providencia stuartii and Klebsiella pneumoniae causes the purple urine bag syndrome.

Detection of antibodies to Ureaplasma urealyticum in pregnant women by enzyme-linked immunosorbent assay using membrane antigen and investigation of the significance of the antibodies.

Detection of genomic variation in Providencia stuartii clinical isolates by analysis of DNA restriction fragment length polymorphisms containing rRNA cistrons.

Possible common biological and immunological properties for detecting encapsulated strains of Staphylococcus epidermidis.

Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probes.

Lack of Shiga-like cytotoxin production by enteroinvasive Escherichia coli.

The reason for loss of agglutinability of Pseudomonas aeruginosa cells heated to 60 degrees C.

Four cases of vesicular lesions in adults caused by enterovirus infections.

DNA hybridization assay for congenital cytomegalovirus infection.

Evaluation of various antibiotics for induction of L forms from Staphylococcus aureus strains isolated from bovine mastitis.

Genetic confirmation of identities of neurotoxigenic Clostridium baratii and Clostridium butyricum implicated as agents of infant botulism.

Isolation of Campylobacter jejuni from the bile of a cholecystic patient.

Modified spin-amplified adsorption procedure with conventional tissue culture tubes for rapid detection and increased recovery of herpes simplex virus from clinical specimens.

Serological responses of patients with lepromatous and tuberculoid leprosy to 30-, 31-, and 32-kilodalton antigens of Mycobacterium tuberculosis.

Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease.

Antithrombin III in the diagnosis of infection in febrile neutropenic patients.

New Borrelia burgdorferi antigenic variant isolated from Ixodes dammini from upstate New York.

Dysgonic fermenter 3 bacteremia in a neutropenic patient with acute lymphocytic leukemia.

Cavitating pneumonia caused by Corynebacterium group JK.

Inadequate mixing in microdilution wells during preparation of antimicrobial dilutions for susceptibility testing.

Addition of monoclonal antibodies specific for Rickettsia akari to the rickettsial diagnostic panel.

Disseminated infection caused by urease-negative Cryptococcus neoformans.

Enzyme profiles of oral spirochetes in RapID-ANA system.

Microparticle enzyme immunoassay for determination of immunoglobulin G antibodies to human cytomegalovirus.

Improved detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli by using a commercial coagglutination test.

Multicenter Comparative Evaluation of Two Rapid Microscopic Methods and Culture for Detection of Chlamydia trachomatis in Patient Specimens

Immunoblot Analysis of Humoral Immune Responses following Infection with Bordetella pertussis or Immunization with Diphtheria-Tetanus-Pertussis Vaccine

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Herpes simplex virus detection by macroscopic reading after overnight incubation and immunoperoxidase staining.

Content Provider	PubMed Central
Author	Ziegler, T. Waris, M. Rautiainen, M. Arstila, P.
Abstract	Human diploid foreskin fibroblast cells grown in 24-well plates were inoculated with clinical specimens by centrifugation at 1,000 X g for 45 min. Cultures were incubated at 37 degrees C overnight, fixed, and stained with peroxidase-labeled monoclonal antibodies against herpes simplex virus types 1 and 2. Stained plaques of infected cells were large enough to be detected with the naked eye, and microscopic examination did not reveal any further positive specimens. The method was compared with standard isolation in human fibroblasts grown in shell vials and inoculated by centrifugation at 4,000 X g, observed microscopically for the occurrence of typical cytopathogenic effect three times a week for 10 days, and then typed by enzyme immunoassay. Of the 289 specimens tested, 105 were positive and 174 were negative by both methods. Six specimens were positive by standard isolation only, two of them containing varicella-zoster virus, and two specimens were stored frozen before being tested by immunoperoxidase staining. Two specimens found negative by standard isolation were positive by immunoperoxidase staining. For two specimens negative by immunoperoxidase staining, the standard isolation cultures were lost due to microbial contamination. Forty-two specimens found positive by standard isolation were clearly positive when stained only 8 h after inoculation. By standard isolation, positive results were reported on the average 3 to 4 days after inoculation, whereas by immunoperoxidase staining the result was available within less than 24 h. Immunoperoxidase staining of infected cells is a sensitive method for rapid laboratory diagnosis of herpes simplex virus infections, and 24-well plates are convenient for the handling of a large number of specimens.
Starting Page	2013
File Format	PDF
ISSN	1098660X
e-ISSN	1098660X
Journal	Journal of Clinical Microbiology
Issue Number	10
Volume Number	26
Language	English
Publisher Date	1988-10-01
Access Restriction	Open
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Microbiology (medical)

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